Combined enzyme mediated fermentation of cellulose and xylose to ethanol by Schizosaccharomyces pombe, cellulase, [beta]-glucosidase, and xylose isomerase
Abstract
A process for producing ethanol from mixed sugar streams from pretreated biomass comprising xylose and cellulose using enzymes to convert these substrates to fermentable sugars; selecting and isolating a yeast Schizosaccharomyces pombe ATCC No. 2476, having the ability to ferment these sugars as they are being formed to produce ethanol; loading the substrates with the fermentation mix composed of yeast, enzymes and substrates; fermenting the loaded substrates and enzymes under anaerobic conditions at a pH range of between about 5.0 to about 6.0 and at a temperature range of between about 35 C to about 40 C until the fermentation is completed, the xylose being isomerized to xylulose, the cellulose being converted to glucose, and these sugars being concurrently converted to ethanol by yeast through means of the anaerobic fermentation; and recovering the ethanol. 2 figures.
- Inventors:
- Issue Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO; Midwest Research Institute
- OSTI Identifier:
- 6671214
- Patent Number(s):
- 5372939 A
- Application Number:
- PPN: US 8-028592
- Assignee:
- Dept. of Energy, Washington, DC (United States) NREL; EDB-95-027813
- DOE Contract Number:
- AC02-83CH10093
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 8 Mar 1993
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; CELLULOSE; ENZYMATIC HYDROLYSIS; ETHANOL; BIOSYNTHESIS; XYLOSE; ANAEROBIC DIGESTION; O-GLYCOSYL HYDROLASES; YEASTS; ALCOHOLS; ALDEHYDES; BIOCONVERSION; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; DIGESTION; ENZYMES; EUMYCOTA; FUNGI; GLYCOSYL HYDROLASES; HYDROLASES; HYDROLYSIS; HYDROXY COMPOUNDS; LYSIS; MANAGEMENT; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; PENTOSES; PLANTS; POLYSACCHARIDES; PROCESSING; PROTEINS; SACCHARIDES; SOLVOLYSIS; SYNTHESIS; WASTE MANAGEMENT; WASTE PROCESSING 090900* -- Biomass Fuels-- Processing-- (1990-)
Citation Formats
Lastick, S.M., Mohagheghi, A., Tucker, M.P., and Grohmann, K. Combined enzyme mediated fermentation of cellulose and xylose to ethanol by Schizosaccharomyces pombe, cellulase, [beta]-glucosidase, and xylose isomerase. United States: N. p., 1994.
Web.
Lastick, S.M., Mohagheghi, A., Tucker, M.P., & Grohmann, K. Combined enzyme mediated fermentation of cellulose and xylose to ethanol by Schizosaccharomyces pombe, cellulase, [beta]-glucosidase, and xylose isomerase. United States.
Lastick, S.M., Mohagheghi, A., Tucker, M.P., and Grohmann, K. Tue .
"Combined enzyme mediated fermentation of cellulose and xylose to ethanol by Schizosaccharomyces pombe, cellulase, [beta]-glucosidase, and xylose isomerase". United States.
@article{osti_6671214,
title = {Combined enzyme mediated fermentation of cellulose and xylose to ethanol by Schizosaccharomyces pombe, cellulase, [beta]-glucosidase, and xylose isomerase},
author = {Lastick, S.M. and Mohagheghi, A. and Tucker, M.P. and Grohmann, K.},
abstractNote = {A process for producing ethanol from mixed sugar streams from pretreated biomass comprising xylose and cellulose using enzymes to convert these substrates to fermentable sugars; selecting and isolating a yeast Schizosaccharomyces pombe ATCC No. 2476, having the ability to ferment these sugars as they are being formed to produce ethanol; loading the substrates with the fermentation mix composed of yeast, enzymes and substrates; fermenting the loaded substrates and enzymes under anaerobic conditions at a pH range of between about 5.0 to about 6.0 and at a temperature range of between about 35 C to about 40 C until the fermentation is completed, the xylose being isomerized to xylulose, the cellulose being converted to glucose, and these sugars being concurrently converted to ethanol by yeast through means of the anaerobic fermentation; and recovering the ethanol. 2 figures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {12}
}