Primary enzyme quantitation
Abstract
The disclosure relates to the quantitation of a primary enzyme concentration by utilizing a substrate for the primary enzyme labeled with a second enzyme which is an indicator enzyme. Enzyme catalysis of the substrate occurs and results in release of the indicator enzyme in an amount directly proportional to the amount of primary enzyme present. By quantifying the free indicator enzyme one determines the amount of primary enzyme present.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 6564779
- Application Number:
- ON: DE83006856
- Assignee:
- ERA-08-018809; EDB-83-052031
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 29 ENERGY PLANNING, POLICY AND ECONOMY; ENZYMES; MEASURING METHODS; CATALYSIS; LABELLED COMPOUNDS; QUANTITY RATIO; 530200* - Environmental-Social Aspects of Energy Technologies- Assessment of Energy Technologies- (-1989)
Citation Formats
Saunders, G C. Primary enzyme quantitation. United States: N. p., 1982.
Web.
Saunders, G C. Primary enzyme quantitation. United States.
Saunders, G C. Thu .
"Primary enzyme quantitation". United States.
@article{osti_6564779,
title = {Primary enzyme quantitation},
author = {Saunders, G C},
abstractNote = {The disclosure relates to the quantitation of a primary enzyme concentration by utilizing a substrate for the primary enzyme labeled with a second enzyme which is an indicator enzyme. Enzyme catalysis of the substrate occurs and results in release of the indicator enzyme in an amount directly proportional to the amount of primary enzyme present. By quantifying the free indicator enzyme one determines the amount of primary enzyme present.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1982},
month = {3}
}