Multiplexed capillary electrophoresis system
Abstract
The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Iowa State Univ., Ames, IA (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 644379
- Patent Number(s):
- 5741411
- Application Number:
- PAN: 8-665,800
- Assignee:
- Iowa State Univ. Research Foundation, Ames, IA (United States)
- DOE Contract Number:
- W-7405-ENG-82
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 21 Apr 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; ELECTROPHORESIS; NUCLEOTIDES; MOLECULAR BIOLOGY
Citation Formats
Yeung, E S, Li, Q, and Lu, X. Multiplexed capillary electrophoresis system. United States: N. p., 1998.
Web.
Yeung, E S, Li, Q, & Lu, X. Multiplexed capillary electrophoresis system. United States.
Yeung, E S, Li, Q, and Lu, X. Tue .
"Multiplexed capillary electrophoresis system". United States.
@article{osti_644379,
title = {Multiplexed capillary electrophoresis system},
author = {Yeung, E S and Li, Q and Lu, X},
abstractNote = {The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {4}
}
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