Time-resolved fluorescence decay measurements for flowing particles
Abstract
Time-resolved fluorescence decay measurements are disclosed for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated CW laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes. 12 figs.
- Inventors:
- Issue Date:
- Sponsoring Org.:
- USDOE; USDOE, Washington, DC (United States)
- OSTI Identifier:
- 6378293
- Patent Number(s):
- 5909278
- Application Number:
- PPN: US 8-902395
- Assignee:
- Univ. of California, Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 29 Jul 1997
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; CELL FLOW SYSTEMS; CYTOLOGICAL TECHNIQUES; FLUORESCENCE SPECTROSCOPY; PLANT CELLS; EMISSION SPECTROSCOPY; SPECTROSCOPY; 400102* - Chemical & Spectral Procedures; 550300 - Cytology
Citation Formats
Deka, C, and Steinkamp, J A. Time-resolved fluorescence decay measurements for flowing particles. United States: N. p., 1999.
Web.
Deka, C, & Steinkamp, J A. Time-resolved fluorescence decay measurements for flowing particles. United States.
Deka, C, and Steinkamp, J A. Tue .
"Time-resolved fluorescence decay measurements for flowing particles". United States.
@article{osti_6378293,
title = {Time-resolved fluorescence decay measurements for flowing particles},
author = {Deka, C and Steinkamp, J A},
abstractNote = {Time-resolved fluorescence decay measurements are disclosed for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated CW laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes. 12 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {6}
}
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