Method for nucleic acid isolation using supercritical fluids
Abstract
A method is disclosed for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification. 4 figs.
- Inventors:
- Issue Date:
- Sponsoring Org.:
- USDOE; USDOE, Washington, DC (United States)
- OSTI Identifier:
- 6377626
- Patent Number(s):
- 5922536
- Application Number:
- PPN: US 8-733816
- Assignee:
- PTO; EDB-99-085946
- DOE Contract Number:
- FG02-95ER82027
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 18 Oct 1996
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; BIODEGRADATION; DNA SEQUENCING; MICROORGANISMS; NUCLEIC ACIDS; SUPERCRITICAL STATE; CHEMICAL REACTIONS; DECOMPOSITION; ORGANIC COMPOUNDS; STRUCTURAL CHEMICAL ANALYSIS; 540220* - Environment, Terrestrial- Chemicals Monitoring & Transport- (1990-)
Citation Formats
Nivens, D E, and Applegate, B M. Method for nucleic acid isolation using supercritical fluids. United States: N. p., 1999.
Web.
Nivens, D E, & Applegate, B M. Method for nucleic acid isolation using supercritical fluids. United States.
Nivens, D E, and Applegate, B M. Tue .
"Method for nucleic acid isolation using supercritical fluids". United States.
@article{osti_6377626,
title = {Method for nucleic acid isolation using supercritical fluids},
author = {Nivens, D E and Applegate, B M},
abstractNote = {A method is disclosed for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {7}
}