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Title: Method for nucleic acid isolation using supercritical fluids

Abstract

A method is disclosed for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification. 4 figs.

Inventors:
;
Issue Date:
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6377626
Patent Number(s):
5922536 A
Application Number:
PPN: US 8-733816
Assignee:
PTO; EDB-99-085946
DOE Contract Number:  
FG02-95ER82027
Resource Type:
Patent
Resource Relation:
Patent File Date: 18 Oct 1996
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; BIODEGRADATION; DNA SEQUENCING; MICROORGANISMS; NUCLEIC ACIDS; SUPERCRITICAL STATE; CHEMICAL REACTIONS; DECOMPOSITION; ORGANIC COMPOUNDS; STRUCTURAL CHEMICAL ANALYSIS; 540220* - Environment, Terrestrial- Chemicals Monitoring & Transport- (1990-)

Citation Formats

Nivens, D.E., and Applegate, B.M. Method for nucleic acid isolation using supercritical fluids. United States: N. p., 1999. Web.
Nivens, D.E., & Applegate, B.M. Method for nucleic acid isolation using supercritical fluids. United States.
Nivens, D.E., and Applegate, B.M. Tue . "Method for nucleic acid isolation using supercritical fluids". United States.
@article{osti_6377626,
title = {Method for nucleic acid isolation using supercritical fluids},
author = {Nivens, D.E. and Applegate, B.M.},
abstractNote = {A method is disclosed for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {7}
}