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Title: Method for distinguishing normal and transformed cells using G1 kinase inhibitors

Abstract

A G[sub 1] phase kinase inhibitor is applied in a low concentration to a population of normal and transformed mammalian cells. The concentration of G[sub 1] phase kinase inhibitor is selected to reversibly arrest normal mammalian cells in the G[sub 1] cell cycle without arresting growth of transformed cells. The transformed cells may then be selectively identified and/or cloned for research or diagnostic purposes. The transformed cells may also be selectively killed by therapeutic agents that do not affect normal cells in the G[sub 1] phase, suggesting that such G[sub 1] phase kinase inhibitors may form an effective adjuvant for use with chemotherapeutic agents in cancer therapy for optimizing the killing dose of chemotherapeutic agents while minimizing undesirable side effects on normal cells.

Inventors:
; ; ;
Issue Date:
OSTI Identifier:
6282503
Patent Number(s):
5185260 A
Application Number:
PPN: US 7-751855
Assignee:
Dept. of Energy, Washington, DC (United States)
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Resource Relation:
Patent File Date: 29 Aug 1991
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; CELL TRANSFORMATIONS; ENZYME INHIBITORS; SPECIFICITY; PHOSPHOTRANSFERASES; CELL CYCLE; CELL KILLING; CHEMOTHERAPY; MAMMALS; NEOPLASMS; ANIMALS; DISEASES; ENZYMES; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PROTEINS; THERAPY; TRANSFERASES; VERTEBRATES; 550200* - Biochemistry

Citation Formats

Crissman, H A, Gadbois, D M, Tobey, R A, and Bradbury, E M. Method for distinguishing normal and transformed cells using G1 kinase inhibitors. United States: N. p., 1993. Web.
Crissman, H A, Gadbois, D M, Tobey, R A, & Bradbury, E M. Method for distinguishing normal and transformed cells using G1 kinase inhibitors. United States.
Crissman, H A, Gadbois, D M, Tobey, R A, and Bradbury, E M. Tue . "Method for distinguishing normal and transformed cells using G1 kinase inhibitors". United States.
@article{osti_6282503,
title = {Method for distinguishing normal and transformed cells using G1 kinase inhibitors},
author = {Crissman, H A and Gadbois, D M and Tobey, R A and Bradbury, E M},
abstractNote = {A G[sub 1] phase kinase inhibitor is applied in a low concentration to a population of normal and transformed mammalian cells. The concentration of G[sub 1] phase kinase inhibitor is selected to reversibly arrest normal mammalian cells in the G[sub 1] cell cycle without arresting growth of transformed cells. The transformed cells may then be selectively identified and/or cloned for research or diagnostic purposes. The transformed cells may also be selectively killed by therapeutic agents that do not affect normal cells in the G[sub 1] phase, suggesting that such G[sub 1] phase kinase inhibitors may form an effective adjuvant for use with chemotherapeutic agents in cancer therapy for optimizing the killing dose of chemotherapeutic agents while minimizing undesirable side effects on normal cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {2}
}