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Title: Method for performing site-specific affinity fractionation for use in DNA sequencing

A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between the cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting the extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to the extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from the array. 14 figs.
Inventors:
; ;
Issue Date:
OSTI Identifier:
6281441
Assignee:
Univ. of Chicago, IL (United States) PTO; EDB-99-081934
Patent Number(s):
US 5905024; A
Application Number:
PPN: US 8-768893
Contract Number:
W-31109-ENG-38
Resource Relation:
Patent File Date: 17 Dec 1996
Sponsoring Org:
USDOE; USDOE, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AFFINITY; DNA; DNA HYBRIDIZATION; DNA SEQUENCING; MOLECULAR STRUCTURE; OLIGONUCLEOTIDES; BIOTECHNOLOGY; GENETIC ENGINEERING; HYBRIDIZATION; NUCLEIC ACID HYBRIDIZATION; NUCLEIC ACIDS; ORGANIC COMPOUNDS; STRUCTURAL CHEMICAL ANALYSIS; 550200* - Biochemistry