Use of continuous/contiguous stacking hybridization as a diagnostic tool
Abstract
A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates. 5 figs.
- Inventors:
- Issue Date:
- Sponsoring Org.:
- USDOE; USDOE, Washington, DC (United States)
- OSTI Identifier:
- 6281146
- Patent Number(s):
- 5908745
- Application Number:
- PPN: US 8-587332
- Assignee:
- Univ. of Chicago, IL (United States)
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 16 Jan 1996
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; ADENINES; CONGENITAL DISEASES; CYTOSINE; DIAGNOSIS; DNA; GUANINE; HEREDITARY DISEASES; OLIGONUCLEOTIDES; THYMINE; AMINES; ANTIMETABOLITES; AROMATICS; AZAARENES; AZINES; DISEASES; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PURINES; PYRIMIDINES; URACILS; 550600* - Medicine; 550900 - Pathology
Citation Formats
Mirzabekov, A D, Yershov, G M, Kirillov, E V, Parinov, S V, Barski, V E, and Lysov, Y P. Use of continuous/contiguous stacking hybridization as a diagnostic tool. United States: N. p., 1999.
Web.
Mirzabekov, A D, Yershov, G M, Kirillov, E V, Parinov, S V, Barski, V E, & Lysov, Y P. Use of continuous/contiguous stacking hybridization as a diagnostic tool. United States.
Mirzabekov, A D, Yershov, G M, Kirillov, E V, Parinov, S V, Barski, V E, and Lysov, Y P. Tue .
"Use of continuous/contiguous stacking hybridization as a diagnostic tool". United States.
@article{osti_6281146,
title = {Use of continuous/contiguous stacking hybridization as a diagnostic tool},
author = {Mirzabekov, A D and Yershov, G M and Kirillov, E V and Parinov, S V and Barski, V E and Lysov, Y P},
abstractNote = {A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates. 5 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {6}
}