Ordered transport and identification of particles
Abstract
A method and apparatus are provided for application of electrical field gradients to induce particle velocities to enable particle sequence and identification information to be obtained. Particle sequence is maintained by providing electroosmotic flow for an electrolytic solution in a particle transport tube. The transport tube and electrolytic solution are selected to provide an electroosmotic radius of >100 so that a plug flow profile is obtained for the electrolytic solution in the transport tube. Thus, particles are maintained in the same order in which they are introduced in the transport tube. When the particles also have known electrophoretic velocities, the field gradients introduce an electrophoretic velocity component onto the electroosmotic velocity. The time that the particles pass selected locations along the transport tube may then be detected and the electrophoretic velocity component calculated for particle identification. One particular application is the ordered transport and identification of labeled nucleotides sequentially cleaved from a strand of DNA.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 6221398
- Patent Number(s):
- 5209834
- Application Number:
- PPN: US 7-848582
- Assignee:
- Dept. of Energy, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 9 Mar 1992
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; MEASURING INSTRUMENTS; DESIGN; NUCLEOTIDES; MEASURING METHODS; DNA SEQUENCING; ELECTRIC FIELDS; ORGANIC COMPOUNDS; STRUCTURAL CHEMICAL ANALYSIS; 550200* - Biochemistry
Citation Formats
Shera, E B. Ordered transport and identification of particles. United States: N. p., 1993.
Web.
Shera, E B. Ordered transport and identification of particles. United States.
Shera, E B. Tue .
"Ordered transport and identification of particles". United States.
@article{osti_6221398,
title = {Ordered transport and identification of particles},
author = {Shera, E B},
abstractNote = {A method and apparatus are provided for application of electrical field gradients to induce particle velocities to enable particle sequence and identification information to be obtained. Particle sequence is maintained by providing electroosmotic flow for an electrolytic solution in a particle transport tube. The transport tube and electrolytic solution are selected to provide an electroosmotic radius of >100 so that a plug flow profile is obtained for the electrolytic solution in the transport tube. Thus, particles are maintained in the same order in which they are introduced in the transport tube. When the particles also have known electrophoretic velocities, the field gradients introduce an electrophoretic velocity component onto the electroosmotic velocity. The time that the particles pass selected locations along the transport tube may then be detected and the electrophoretic velocity component calculated for particle identification. One particular application is the ordered transport and identification of labeled nucleotides sequentially cleaved from a strand of DNA.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {5}
}