Cloning and expression of the gene for bacteriophage T7 RNA polymerase
Abstract
This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the T7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 6168827
- Application Number:
- ON: TI85005752
- Assignee:
- Dept of Energy EDB-85-039823
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; BACTERIOPHAGES; GENETIC ENGINEERING; GENES; CLONING; RNA POLYMERASES; BIOSYNTHESIS; PLASMIDS; CELL CONSTITUENTS; ENZYMES; MICROORGANISMS; NUCLEOTIDYLTRANSFERASES; PARASITES; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; SYNTHESIS; TRANSFERASES; VIRUSES; 550200* - Biochemistry
Citation Formats
Studier, F.W., Davanloo, P., and Rosenberg, A.H. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. United States: N. p., 1984.
Web.
Studier, F.W., Davanloo, P., & Rosenberg, A.H. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. United States.
Studier, F.W., Davanloo, P., and Rosenberg, A.H. Fri .
"Cloning and expression of the gene for bacteriophage T7 RNA polymerase". United States.
@article{osti_6168827,
title = {Cloning and expression of the gene for bacteriophage T7 RNA polymerase},
author = {Studier, F.W. and Davanloo, P. and Rosenberg, A.H.},
abstractNote = {This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the T7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1984},
month = {3}
}