Improved flow cytometer measurement of binding assays
Abstract
The invention relates to a method of measuring binding assays carried out with different size particles wherein the binding assay sample is run through a flow cytometer without separating the sample from the marking agent. The amount of a binding reactant present in a sample is determined by providing particles with a coating of binder and also a known quantity of smaller particles with a coating of binder reactant. The binding reactant is the same as the binding reactant present in the sample. The smaller particles also contain a fluorescent chemical. The particles are combined with the sample and the binding reaction is allowed to occur for a set length of time followed by combining the smaller particles with the mixture of the particles and the sample produced and allowing the binding reactions to proceed to equilibrium. The fluorescence and light scatter of the combined mixture is then measured as the combined mixture passes through a flow cytometer equipped with a laser to bring about fluorescence, and the number and strength of fluorescent events are compared. A similar method is also provided for determining the amount of antigen present in the sample by providing spheres with an antibody coating andmore »
- Inventors:
- Issue Date:
- OSTI Identifier:
- 6113747
- Application Number:
- ON: TI85006407
- Assignee:
- Dept. of Energy
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Resource Relation:
- Other Information: Portions are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; CYTOCHEMISTRY; MEASURING INSTRUMENTS; MICROSPHERES; BIOCHEMISTRY; CHEMISTRY; 440300* - Miscellaneous Instruments- (-1989)
Citation Formats
Saunders, G C. Improved flow cytometer measurement of binding assays. United States: N. p., 1984.
Web.
Saunders, G C. Improved flow cytometer measurement of binding assays. United States.
Saunders, G C. Wed .
"Improved flow cytometer measurement of binding assays". United States.
@article{osti_6113747,
title = {Improved flow cytometer measurement of binding assays},
author = {Saunders, G C},
abstractNote = {The invention relates to a method of measuring binding assays carried out with different size particles wherein the binding assay sample is run through a flow cytometer without separating the sample from the marking agent. The amount of a binding reactant present in a sample is determined by providing particles with a coating of binder and also a known quantity of smaller particles with a coating of binder reactant. The binding reactant is the same as the binding reactant present in the sample. The smaller particles also contain a fluorescent chemical. The particles are combined with the sample and the binding reaction is allowed to occur for a set length of time followed by combining the smaller particles with the mixture of the particles and the sample produced and allowing the binding reactions to proceed to equilibrium. The fluorescence and light scatter of the combined mixture is then measured as the combined mixture passes through a flow cytometer equipped with a laser to bring about fluorescence, and the number and strength of fluorescent events are compared. A similar method is also provided for determining the amount of antigen present in the sample by providing spheres with an antibody coating and some smaller spheres with an antigen coating. (LEW)},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed May 30 00:00:00 EDT 1984},
month = {Wed May 30 00:00:00 EDT 1984}
}