skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Apparatus for eliminating background interference in fluorescence measurements

Abstract

The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser which excites a different stained component of the same biological particle.

Inventors:
;
Issue Date:
OSTI Identifier:
6111956
Application Number:
ON: TI85006515
Assignee:
Dept. of Energy EDB-85-039845
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL FLOW SYSTEMS; BACKGROUND NOISE; FLUORESCENCE SPECTROSCOPY; LASERS; SIGNAL-TO-NOISE RATIO; EMISSION SPECTROSCOPY; NOISE; SPECTROSCOPY; 550300* - Cytology

Citation Formats

Martin, J.C., and Jett, J.H. Apparatus for eliminating background interference in fluorescence measurements. United States: N. p., 1984. Web.
Martin, J.C., & Jett, J.H. Apparatus for eliminating background interference in fluorescence measurements. United States.
Martin, J.C., and Jett, J.H. Fri . "Apparatus for eliminating background interference in fluorescence measurements". United States.
@article{osti_6111956,
title = {Apparatus for eliminating background interference in fluorescence measurements},
author = {Martin, J.C. and Jett, J.H.},
abstractNote = {The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser which excites a different stained component of the same biological particle.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1984},
month = {1}
}