Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes
Abstract
Apparatus and method is described for measuring intensities at a plurality of wavelengths and lifetimes. A source of multiple-wavelength electromagnetic radiation is passed through a first interferometer modulated at a first frequency, the output thereof being directed into a sample to be investigated. The light emitted from the sample as a result of the interaction thereof with the excitation radiation is directed into a second interferometer modulated at a second frequency, and the output detected and analyzed. In this manner excitation, emission, and lifetime information may be obtained for a multiplicity of fluorochromes in the sample.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 6047614
- Patent Number(s):
- 5208651
- Application Number:
- PPN: US 7-731070
- Assignee:
- Univ. of California, Alameda, CA (United States)
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 16 Jul 1991
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 47 OTHER INSTRUMENTATION; INTERFEROMETERS; DESIGN; MATERIALS; FLUORESCENCE; ELECTROMAGNETIC RADIATION; EXCITATION; FREQUENCY DEPENDENCE; LIFETIME; MODULATION; OPERATION; WAVELENGTHS; ENERGY-LEVEL TRANSITIONS; LUMINESCENCE; MEASURING INSTRUMENTS; RADIATIONS; 400102* - Chemical & Spectral Procedures; 440800 - Miscellaneous Instrumentation- (1990-)
Citation Formats
Buican, T N. Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes. United States: N. p., 1993.
Web.
Buican, T N. Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes. United States.
Buican, T N. Tue .
"Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes". United States.
@article{osti_6047614,
title = {Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes},
author = {Buican, T N},
abstractNote = {Apparatus and method is described for measuring intensities at a plurality of wavelengths and lifetimes. A source of multiple-wavelength electromagnetic radiation is passed through a first interferometer modulated at a first frequency, the output thereof being directed into a sample to be investigated. The light emitted from the sample as a result of the interaction thereof with the excitation radiation is directed into a second interferometer modulated at a second frequency, and the output detected and analyzed. In this manner excitation, emission, and lifetime information may be obtained for a multiplicity of fluorochromes in the sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 04 00:00:00 EDT 1993},
month = {Tue May 04 00:00:00 EDT 1993}
}
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