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Title: Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes

Abstract

Apparatus and method is described for measuring intensities at a plurality of wavelengths and lifetimes. A source of multiple-wavelength electromagnetic radiation is passed through a first interferometer modulated at a first frequency, the output thereof being directed into a sample to be investigated. The light emitted from the sample as a result of the interaction thereof with the excitation radiation is directed into a second interferometer modulated at a second frequency, and the output detected and analyzed. In this manner excitation, emission, and lifetime information may be obtained for a multiplicity of fluorochromes in the sample.

Inventors:
Issue Date:
OSTI Identifier:
6047614
Patent Number(s):
5208651 A
Application Number:
PPN: US 7-731070
Assignee:
Univ. of California, Alameda, CA (United States)
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Resource Relation:
Patent File Date: 16 Jul 1991
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 47 OTHER INSTRUMENTATION; INTERFEROMETERS; DESIGN; MATERIALS; FLUORESCENCE; ELECTROMAGNETIC RADIATION; EXCITATION; FREQUENCY DEPENDENCE; LIFETIME; MODULATION; OPERATION; WAVELENGTHS; ENERGY-LEVEL TRANSITIONS; LUMINESCENCE; MEASURING INSTRUMENTS; RADIATIONS; 400102* - Chemical & Spectral Procedures; 440800 - Miscellaneous Instrumentation- (1990-)

Citation Formats

Buican, T N. Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes. United States: N. p., 1993. Web.
Buican, T N. Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes. United States.
Buican, T N. Tue . "Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes". United States.
@article{osti_6047614,
title = {Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes},
author = {Buican, T N},
abstractNote = {Apparatus and method is described for measuring intensities at a plurality of wavelengths and lifetimes. A source of multiple-wavelength electromagnetic radiation is passed through a first interferometer modulated at a first frequency, the output thereof being directed into a sample to be investigated. The light emitted from the sample as a result of the interaction thereof with the excitation radiation is directed into a second interferometer modulated at a second frequency, and the output detected and analyzed. In this manner excitation, emission, and lifetime information may be obtained for a multiplicity of fluorochromes in the sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {5}
}