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Title: Nucleic acid isolation

Abstract

A method is provided for isolating DNA from eukaryotic cell and flow sorted chromosomes. When DNA is removed from chromosome and cell structure, detergent and proteolytic digestion products remain with the DNA. These products can be removed with organic extraction, but the process steps associated with organic extraction reduces the size of DNA fragments available for experimental use. The present process removes the waste products by dialyzing a solution containing the DNA against a solution containing polyethylene glycol (PEG). The waste products dialyze into the PEG leaving isolated DNA. The remaining DNA has been prepared with fragments containing more than 160 kb. The isolated DNA has been used in conventional protocols without effect on the protocol.

Inventors:
; ;
Issue Date:
Research Org.:
Los Alamos National Lab., NM (USA)
OSTI Identifier:
6041312
Patent Number(s):
-US-A7146557
Application Number:
ON: DE89010885
Assignee:
Dept. of Energy TIC; ERA-14-029377; EDB-89-079737
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; CHROMOSOMES; SEPARATION PROCESSES; DNA; DIALYSIS; INVENTIONS; POLYETHYLENE GLYCOLS; ALCOHOLS; GLYCOLS; HYDROXY COMPOUNDS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC POLYMERS; POLYMERS; 500200* - Environment, Atmospheric- Chemicals Monitoring & Transport- (-1989)

Citation Formats

Longmire, J.L., Lewis, A.K., and Hildebrand, C.E. Nucleic acid isolation. United States: N. p., 1988. Web.
Longmire, J.L., Lewis, A.K., & Hildebrand, C.E. Nucleic acid isolation. United States.
Longmire, J.L., Lewis, A.K., and Hildebrand, C.E. Thu . "Nucleic acid isolation". United States.
@article{osti_6041312,
title = {Nucleic acid isolation},
author = {Longmire, J.L. and Lewis, A.K. and Hildebrand, C.E.},
abstractNote = {A method is provided for isolating DNA from eukaryotic cell and flow sorted chromosomes. When DNA is removed from chromosome and cell structure, detergent and proteolytic digestion products remain with the DNA. These products can be removed with organic extraction, but the process steps associated with organic extraction reduces the size of DNA fragments available for experimental use. The present process removes the waste products by dialyzing a solution containing the DNA against a solution containing polyethylene glycol (PEG). The waste products dialyze into the PEG leaving isolated DNA. The remaining DNA has been prepared with fragments containing more than 160 kb. The isolated DNA has been used in conventional protocols without effect on the protocol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {1}
}