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Title: Process of labeling specific chromosomes using recombinant repetitive DNA

Abstract

Chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family members and consensus sequences of the repetitive DNA families for the chromosome preferential sequences. The selected low homology regions are then hybridized with chromosomes to determine those low homology regions hybridized with a specific chromosome under normal stringency conditions.

Inventors:
;
Issue Date:
Research Org.:
Los Alamos National Lab., NM (USA)
OSTI Identifier:
6040189
Patent Number(s):
-US-A7155451
Application Number:
ON: DE89011800
Assignee:
Dept. of Energy TIC; EDB-89-079886
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHROMOSOMES; CLASSIFICATION; RECOMBINANT DNA; LABELLING; INVENTIONS; DNA; NUCLEIC ACIDS; ORGANIC COMPOUNDS; 550200* - Biochemistry

Citation Formats

Moyzis, R.K., and Meyne, J. Process of labeling specific chromosomes using recombinant repetitive DNA. United States: N. p., 1988. Web.
Moyzis, R.K., & Meyne, J. Process of labeling specific chromosomes using recombinant repetitive DNA. United States.
Moyzis, R.K., and Meyne, J. Fri . "Process of labeling specific chromosomes using recombinant repetitive DNA". United States.
@article{osti_6040189,
title = {Process of labeling specific chromosomes using recombinant repetitive DNA},
author = {Moyzis, R.K. and Meyne, J.},
abstractNote = {Chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family members and consensus sequences of the repetitive DNA families for the chromosome preferential sequences. The selected low homology regions are then hybridized with chromosomes to determine those low homology regions hybridized with a specific chromosome under normal stringency conditions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {2}
}