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Title: Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions

Abstract

A method is provided for detecting nucleic acid sequence aberrations using two immobilization steps. According to the method, a nucleic acid sequence aberration is detected by detecting nucleic acid sequences having both a first nucleic acid sequence type (e.g., from a first chromosome) and a second nucleic acid sequence type (e.g., from a second chromosome), the presence of the first and the second nucleic acid sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. In the method, immobilization of a first hybridization probe is used to isolate a first set of nucleic acids in the sample which contain the first nucleic acid sequence type. Immobilization of a second hybridization probe is then used to isolate a second set of nucleic acids from within the first set of nucleic acids which contain the second nucleic acid sequence type. The second set of nucleic acids are then detected, their presence indicating the presence of a nucleic acid sequence aberration. 14 figs.

Inventors:
; ;
Issue Date:
Research Org.:
Univ. of California (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
597115
Patent Number(s):
5731153
Application Number:
PAN: 8-703,302
Assignee:
Univ. of California, Oakland, CA (United States)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 24 Mar 1998
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; CHROMOSOMAL ABERRATIONS; NUCLEIC ACID HYBRIDIZATION; MEASURING METHODS; CHROMOSOMES; AMINO ACID SEQUENCE

Citation Formats

Lucas, J N, Straume, T, and Bogen, K T. Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions. United States: N. p., 1998. Web.
Lucas, J N, Straume, T, & Bogen, K T. Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions. United States.
Lucas, J N, Straume, T, and Bogen, K T. Tue . "Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions". United States.
@article{osti_597115,
title = {Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions},
author = {Lucas, J N and Straume, T and Bogen, K T},
abstractNote = {A method is provided for detecting nucleic acid sequence aberrations using two immobilization steps. According to the method, a nucleic acid sequence aberration is detected by detecting nucleic acid sequences having both a first nucleic acid sequence type (e.g., from a first chromosome) and a second nucleic acid sequence type (e.g., from a second chromosome), the presence of the first and the second nucleic acid sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. In the method, immobilization of a first hybridization probe is used to isolate a first set of nucleic acids in the sample which contain the first nucleic acid sequence type. Immobilization of a second hybridization probe is then used to isolate a second set of nucleic acids from within the first set of nucleic acids which contain the second nucleic acid sequence type. The second set of nucleic acids are then detected, their presence indicating the presence of a nucleic acid sequence aberration. 14 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {3}
}