Sequence independent amplification of DNA
Abstract
The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Chicago, IL (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)
- OSTI Identifier:
- 597114
- Patent Number(s):
- 5731171
- Application Number:
- PAN: 8-096,637; CNN: Grant CA49133;CA42557;CA40046
- Assignee:
- Arch Development Corp., Chicago, IL (United States)
- DOE Contract Number:
- FG02-86ER60408
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 24 Mar 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCERS; GENE AMPLIFICATION; DNA SEQUENCING; CHROMOSOMES; IN-SITU HYBRIDIZATION
Citation Formats
Bohlander, S K. Sequence independent amplification of DNA. United States: N. p., 1998.
Web.
Bohlander, S K. Sequence independent amplification of DNA. United States.
Bohlander, S K. Tue .
"Sequence independent amplification of DNA". United States.
@article{osti_597114,
title = {Sequence independent amplification of DNA},
author = {Bohlander, S K},
abstractNote = {The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {3}
}
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