Method for increasing thermostability in cellulase ennzymes
Abstract
The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product. 8 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 570396
- Patent Number(s):
- 5712142
- Application Number:
- PAN: 8-604,913
- Assignee:
- Midwest Research Inst., Kansas City, MO (United States)
- DOE Contract Number:
- AC36-83CH10093
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 27 Jan 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; CELLULASE; GENE REGULATION; STABILITY; RECEPTORS; GENES
Citation Formats
Adney, W S, Thomas, S R, Baker, J O, Himmel, M E, and Chou, Y C. Method for increasing thermostability in cellulase ennzymes. United States: N. p., 1998.
Web.
Adney, W S, Thomas, S R, Baker, J O, Himmel, M E, & Chou, Y C. Method for increasing thermostability in cellulase ennzymes. United States.
Adney, W S, Thomas, S R, Baker, J O, Himmel, M E, and Chou, Y C. Tue .
"Method for increasing thermostability in cellulase ennzymes". United States.
@article{osti_570396,
title = {Method for increasing thermostability in cellulase ennzymes},
author = {Adney, W S and Thomas, S R and Baker, J O and Himmel, M E and Chou, Y C},
abstractNote = {The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product. 8 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}
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