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Title: Method for increasing thermostability in cellulase ennzymes

Abstract

The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product. 8 figs.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Midwest Research Institute, Kansas City, MO (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
570396
Patent Number(s):
5712142
Application Number:
PAN: 8-604,913
Assignee:
Midwest Research Inst., Kansas City, MO (United States)
DOE Contract Number:  
AC36-83CH10093
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 27 Jan 1998
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; CELLULASE; GENE REGULATION; STABILITY; RECEPTORS; GENES

Citation Formats

Adney, W S, Thomas, S R, Baker, J O, Himmel, M E, and Chou, Y C. Method for increasing thermostability in cellulase ennzymes. United States: N. p., 1998. Web.
Adney, W S, Thomas, S R, Baker, J O, Himmel, M E, & Chou, Y C. Method for increasing thermostability in cellulase ennzymes. United States.
Adney, W S, Thomas, S R, Baker, J O, Himmel, M E, and Chou, Y C. Tue . "Method for increasing thermostability in cellulase ennzymes". United States.
@article{osti_570396,
title = {Method for increasing thermostability in cellulase ennzymes},
author = {Adney, W S and Thomas, S R and Baker, J O and Himmel, M E and Chou, Y C},
abstractNote = {The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product. 8 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}