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Title: Surface enhanced Raman gene probe and methods thereof

Abstract

The subject invention disclosed is a new gene probe biosensor and methods based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays. 18 figs.

Inventors:
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
570382
Patent Number(s):
5721102
Application Number:
PAN: 8-792,085
Assignee:
Lockheed Martin Energy Systems, Inc., Oak Ridge, TN (United States)
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 24 Feb 1998
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA HYBRIDIZATION; OLIGONUCLEOTIDES; GENES; MOLECULAR BIOLOGY; RAMAN SPECTRA

Citation Formats

Vo-Dinh, T. Surface enhanced Raman gene probe and methods thereof. United States: N. p., 1998. Web.
Vo-Dinh, T. Surface enhanced Raman gene probe and methods thereof. United States.
Vo-Dinh, T. Tue . "Surface enhanced Raman gene probe and methods thereof". United States.
@article{osti_570382,
title = {Surface enhanced Raman gene probe and methods thereof},
author = {Vo-Dinh, T},
abstractNote = {The subject invention disclosed is a new gene probe biosensor and methods based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays. 18 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {2}
}

Works referenced in this record:

Surface raman spectroelectrochemistry: Part I. Heterocyclic, aromatic, and aliphatic amines adsorbed on the anodized silver electrode
journal, November 1977


Surface-Enhanced Raman Gene Probes
journal, October 1994


Charge-Coupled Device Fluorescence Detection for Capillary-Zone Electrophoresis (CCD-CZE)
journal, June 1990


Selective surface-enhanced Raman spectroscopy using a polymer-coated substrate
journal, September 1995