Surface enhanced Raman gene probe and methods thereof
Abstract
The subject invention disclosed is a new gene probe biosensor and methods based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays. 18 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 570382
- Patent Number(s):
- 5721102
- Application Number:
- PAN: 8-792,085
- Assignee:
- Lockheed Martin Energy Systems, Inc., Oak Ridge, TN (United States)
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 24 Feb 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA HYBRIDIZATION; OLIGONUCLEOTIDES; GENES; MOLECULAR BIOLOGY; RAMAN SPECTRA
Citation Formats
Vo-Dinh, T. Surface enhanced Raman gene probe and methods thereof. United States: N. p., 1998.
Web.
Vo-Dinh, T. Surface enhanced Raman gene probe and methods thereof. United States.
Vo-Dinh, T. Tue .
"Surface enhanced Raman gene probe and methods thereof". United States.
@article{osti_570382,
title = {Surface enhanced Raman gene probe and methods thereof},
author = {Vo-Dinh, T},
abstractNote = {The subject invention disclosed is a new gene probe biosensor and methods based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays. 18 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {2}
}
Search for the full text at the U.S. Patent and Trademark Office
Note: You will be redirected to the USPTO site, which may require a pop-up blocker to be deactivated to view the patent. If so, you will need to manually turn off your browser's pop-up blocker, typically found within the browser settings. (See DOE Patents FAQs for more information.)
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Surface raman spectroelectrochemistry: Part I. Heterocyclic, aromatic, and aliphatic amines adsorbed on the anodized silver electrode
journal, November 1977
- Jeanmaire, David L.; Van Duyne, Richard P.
- Journal of Electroanalytical Chemistry and Interfacial Electrochemistry, Vol. 84, Issue 1, p. 1-20
Wet chemical approaches to the characterization of organic surfaces: self-assembled monolayers, wetting, and the physical-organic chemistry of the solid-liquid interface
journal, January 1990
- Whitesides, George M.; Laibinis, Paul E.
- Langmuir, Vol. 6, Issue 1
Surface-Enhanced Raman Gene Probes
journal, October 1994
- Vo-Dinh, T.; Houck, K.; Stokes, D. L.
- Analytical Chemistry, Vol. 66, Issue 20
Charge-Coupled Device Fluorescence Detection for Capillary-Zone Electrophoresis (CCD-CZE)
journal, June 1990
- Cheng, Yung-Fong; Piccard, Richard D.; Vo-Dinh, Tuan
- Applied Spectroscopy, Vol. 44, Issue 5
Selective surface-enhanced Raman spectroscopy using a polymer-coated substrate
journal, September 1995
- Pal, Anjali.; Stokes, David L.; Alarie, Jean Pierre.
- Analytical Chemistry, Vol. 67, Issue 18