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Title: Cloning and expression of the gene for bacteriophage T7 RNA polymerase

Abstract

This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells. 10 figs.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Associated Universities Inc
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
563682
Patent Number(s):
5,693,489
Application Number:
PAN: 8-259,560
Assignee:
Associated Universities, Inc., Washington, DC (United States) PTO; SCA: 550200; PA: EDB-98:016246; SN: 98001896330
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 2 Dec 1997
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; POLYMERASES; GENE REGULATION; BACTERIOPHAGES; GENES

Citation Formats

Studier, F.W., Davanloo, P., Rosenberg, A.H., Moffatt, B.A., and Dunn, J.J. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. United States: N. p., 1997. Web.
Studier, F.W., Davanloo, P., Rosenberg, A.H., Moffatt, B.A., & Dunn, J.J. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. United States.
Studier, F.W., Davanloo, P., Rosenberg, A.H., Moffatt, B.A., and Dunn, J.J. Tue . "Cloning and expression of the gene for bacteriophage T7 RNA polymerase". United States.
@article{osti_563682,
title = {Cloning and expression of the gene for bacteriophage T7 RNA polymerase},
author = {Studier, F.W. and Davanloo, P. and Rosenberg, A.H. and Moffatt, B.A. and Dunn, J.J.},
abstractNote = {This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells. 10 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {12}
}