Cloning and expression of the gene for bacteriophage T7 RNA polymerase
Abstract
This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells. 10 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Associated Universities Inc
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 563682
- Patent Number(s):
- 5693489
- Application Number:
- PAN: 8-259,560
- Assignee:
- Associated Universities, Inc., Washington, DC (United States)
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 2 Dec 1997
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; POLYMERASES; GENE REGULATION; BACTERIOPHAGES; GENES
Citation Formats
Studier, F W, Davanloo, P, Rosenberg, A H, Moffatt, B A, and Dunn, J J. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. United States: N. p., 1997.
Web.
Studier, F W, Davanloo, P, Rosenberg, A H, Moffatt, B A, & Dunn, J J. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. United States.
Studier, F W, Davanloo, P, Rosenberg, A H, Moffatt, B A, and Dunn, J J. Tue .
"Cloning and expression of the gene for bacteriophage T7 RNA polymerase". United States.
@article{osti_563682,
title = {Cloning and expression of the gene for bacteriophage T7 RNA polymerase},
author = {Studier, F W and Davanloo, P and Rosenberg, A H and Moffatt, B A and Dunn, J J},
abstractNote = {This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells. 10 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 02 00:00:00 EST 1997},
month = {Tue Dec 02 00:00:00 EST 1997}
}