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Title: Capillaries for use in a multiplexed capillary electrophoresis system

Abstract

The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.

Inventors:
; ;
Issue Date:
Research Org.:
Iowa State University
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
563680
Patent Number(s):
5,695,626
Application Number:
PAN: 8-667,950
Assignee:
Iowa State Univ. Research Foundation, Ames, IA (United States) PTO; SCA: 550200; PA: EDB-98:016258; SN: 98001896320
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 9 Dec 1997
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ELECTROPHORESIS; DNA SEQUENCERS; DESIGN; DNA SEQUENCING; NUCLEOTIDES

Citation Formats

Yeung, E.S., Chang, H.T., and Fung, E.N. Capillaries for use in a multiplexed capillary electrophoresis system. United States: N. p., 1997. Web.
Yeung, E.S., Chang, H.T., & Fung, E.N. Capillaries for use in a multiplexed capillary electrophoresis system. United States.
Yeung, E.S., Chang, H.T., and Fung, E.N. Tue . "Capillaries for use in a multiplexed capillary electrophoresis system". United States.
@article{osti_563680,
title = {Capillaries for use in a multiplexed capillary electrophoresis system},
author = {Yeung, E.S. and Chang, H.T. and Fung, E.N.},
abstractNote = {The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {12}
}