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Title: Procedure for normalization of cDNA libraries

Abstract

This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library. 1 fig.

Inventors:
;
Issue Date:
Research Org.:
Columbia University
Sponsoring Org.:
National Insts. of Health, Bethesda, MD (United States); Department of Health and Human Services, Washington, DC (United States); USDOE, Washington, DC (United States)
OSTI Identifier:
563674
Patent Number(s):
5,702,898
Application Number:
PAN: 8-465,857; CNN: Grant R01-HG00980
Assignee:
Columbia Univ., New York, NY (United States) PTO; SCA: 550200; PA: EDB-98:016247; SN: 98001896298
DOE Contract Number:  
FG02-91ER61233
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 30 Dec 1997
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; MOLECULAR BIOLOGY; MOLECULAR STRUCTURE; RECOMBINANT DNA; BIOTECHNOLOGY

Citation Formats

Bonaldo, M.D., and Soares, M.B. Procedure for normalization of cDNA libraries. United States: N. p., 1997. Web.
Bonaldo, M.D., & Soares, M.B. Procedure for normalization of cDNA libraries. United States.
Bonaldo, M.D., and Soares, M.B. Tue . "Procedure for normalization of cDNA libraries". United States.
@article{osti_563674,
title = {Procedure for normalization of cDNA libraries},
author = {Bonaldo, M.D. and Soares, M.B.},
abstractNote = {This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library. 1 fig.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {12}
}