Procedure for normalization of cDNA libraries

Bonaldo, M D; Soares, M B

Title: Procedure for normalization of cDNA libraries

Full Record
Other Related Research

Abstract

This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library. 1 fig.

Inventors:: Bonaldo, M D; Soares, M B

Issue Date:: 1997-12-30

Research Org.:: Columbia Univ., New York, NY (United States)

Sponsoring Org.:: National Insts. of Health, Bethesda, MD (United States); Department of Health and Human Services, Washington, DC (United States); USDOE, Washington, DC (United States)

OSTI Identifier:: 563674

Patent Number(s):: 5702898

Application Number:: PAN: 8-465,857; CNN: Grant R01-HG00980

Assignee:: Columbia Univ., New York, NY (United States)

DOE Contract Number:: FG02-91ER61233

Resource Type:: Patent

Resource Relation:: Other Information: PBD: 30 Dec 1997

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; MOLECULAR BIOLOGY; MOLECULAR STRUCTURE; RECOMBINANT DNA; BIOTECHNOLOGY

Citation Formats


                    Bonaldo, M D, and Soares, M B. Procedure for normalization of cDNA libraries.  United States: N. p., 1997. 
        Web.

Copy to clipboard


                    Bonaldo, M D, & Soares, M B. Procedure for normalization of cDNA libraries.  United States.

Copy to clipboard


                    Bonaldo, M D, and Soares, M B. Tue .  
        "Procedure for normalization of cDNA libraries".  United States.

Copy to clipboard


                    
@article{osti_563674,

  title        = {Procedure for normalization of cDNA libraries},

  author       = {Bonaldo, M D and Soares, M B},

  abstractNote = {This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library. 1 fig.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1997},

  month        = {12}

}

Copy to clipboard

Patent:

Search for the full text at the U.S. Patent and Trademark Office

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE Patents and OSTI.GOV collections:

Procedure for normalization of cDNA libraries

Patent Bonaldo, Maria DeFatima [New York, NY]; Soares, Marcelo Bento [New York, NY]

This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.
Full Text Available
Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs

Patent Soares, M B; Fatima Bonaldo, M de

This invention provides a method to normalize a cDNA library comprising: (a) constructing a directionally cloned library containing cDNA inserts wherein the insert is capable of being amplified by polymerase chain reaction; (b) converting a double-stranded cDNA library into single-stranded DNA circles; (c) generating single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) by polymerase chain reaction with appropriate primers; (d) hybridizing the single-stranded DNA circles converted in step (b) with the complementary single-stranded nucleic acid molecules generated in step (c) to produce partial duplexes to an appropriate Cot; and (e) separating the unhybridized single-strandedmore » « less
Normalized cDNA libraries

Patent Soares, M B; Efstratiadis, A

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.
Method for construction of normalized cDNA libraries

Patent Soares, M B; Efstratiadis, A

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form. The method comprises: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.
Method for construction of normalized cDNA libraries

Patent Soares, M B; Efstratiadis, A

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries. 19more » figs. « less

Similar Records