Nucleotide sequences encoding a thermostable alkaline protease

Wilson, D B; Lao, G

Title: Nucleotide sequences encoding a thermostable alkaline protease

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Abstract

Nucleotide sequences, derived from a thermophilic actinomycete microorganism, which encode a thermostable alkaline protease are disclosed. Also disclosed are variants of the nucleotide sequences which encode a polypeptide having thermostable alkaline proteolytic activity. Recombinant thermostable alkaline protease or recombinant polypeptide may be obtained by culturing in a medium a host cell genetically engineered to contain and express a nucleotide sequence according to the present invention, and recovering the recombinant thermostable alkaline protease or recombinant polypeptide from the culture medium. 3 figs.

Inventors:: Wilson, D B; Lao, G

Issue Date:: 1998-01-06

Research Org.:: Cornell Univ., Ithaca, NY (United States)

Sponsoring Org.:: USDOE, Washington, DC (United States)

OSTI Identifier:: 563671

Patent Number(s):: 5705379

Application Number:: PAN: 8-736,361

Assignee:: Cornell Research Foundation, Inc., Ithaca, NY (United States)

DOE Contract Number:: FG02-84ER13233

Resource Type:: Patent

Resource Relation:: Other Information: PBD: 6 Jan 1998

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; AMINO ACID SEQUENCE; PEPTIDE HYDROLASES; POLYPEPTIDES; GENETIC ENGINEERING; FUNGI

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                    Wilson, D B, and Lao, G. Nucleotide sequences encoding a thermostable alkaline protease.  United States: N. p., 1998. 
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                    Wilson, D B, & Lao, G. Nucleotide sequences encoding a thermostable alkaline protease.  United States.

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                    Wilson, D B, and Lao, G. Tue .  
        "Nucleotide sequences encoding a thermostable alkaline protease".  United States.

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@article{osti_563671,

  title        = {Nucleotide sequences encoding a thermostable alkaline protease},

  author       = {Wilson, D B and Lao, G},

  abstractNote = {Nucleotide sequences, derived from a thermophilic actinomycete microorganism, which encode a thermostable alkaline protease are disclosed. Also disclosed are variants of the nucleotide sequences which encode a polypeptide having thermostable alkaline proteolytic activity. Recombinant thermostable alkaline protease or recombinant polypeptide may be obtained by culturing in a medium a host cell genetically engineered to contain and express a nucleotide sequence according to the present invention, and recovering the recombinant thermostable alkaline protease or recombinant polypeptide from the culture medium. 3 figs.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {1}

}

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Nucleotide sequences, derived from a thermophilic actinomycete microorganism, which encode a thermostable alkaline protease are disclosed. Also disclosed are variants of the nucleotide sequences which encode a polypeptide having thermostable alkaline proteolytic activity. Recombinant thermostable alkaline protease or recombinant polypeptide may be obtained by culturing in a medium a host cell genetically engineered to contain and express a nucleotide sequence according to the present invention, and recovering the recombinant thermostable alkaline protease or recombinant polypeptide from the culture medium.
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