Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof
Abstract
The present invention provides monoclonal antibodies specific to and distinguishing between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype. 4 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 5577044
- Application Number:
- ON: DE86013864
- Assignee:
- Dept. of Energy
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; HEMOGLOBIN; ANTIGEN-ANTIBODY REACTIONS; HYBRIDOMAS; DESIGN; SICKLE CELL ANEMIA; DIAGNOSTIC TECHNIQUES; CELL FLOW SYSTEMS; FLUORESCENCE; MONOCLONAL ANTIBODIES; ANEMIAS; ANIMAL CELLS; ANTIBODIES; CARBOXYLIC ACIDS; DISEASES; GLOBIN; HEMIC DISEASES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; LUMINESCENCE; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PIGMENTS; PORPHYRINS; PROTEINS; SYMPTOMS; 550900* - Pathology
Citation Formats
Jensen, R H, Vanderlaan, M, Bigbee, W L, Stanker, L H, Branscomb, E W, and Grabske, R J. Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof. United States: N. p., 1984.
Web.
Jensen, R H, Vanderlaan, M, Bigbee, W L, Stanker, L H, Branscomb, E W, & Grabske, R J. Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof. United States.
Jensen, R H, Vanderlaan, M, Bigbee, W L, Stanker, L H, Branscomb, E W, and Grabske, R J. Thu .
"Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof". United States.
@article{osti_5577044,
title = {Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof},
author = {Jensen, R H and Vanderlaan, M and Bigbee, W L and Stanker, L H and Branscomb, E W and Grabske, R J},
abstractNote = {The present invention provides monoclonal antibodies specific to and distinguishing between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1984},
month = {11}
}