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Title: pLS101 plasmid vector

Abstract

Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb ma1M gene fragment ligated to a 4.4 Kb Tcr DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems. 5 figs., 2 tabs.

Inventors:
;
Issue Date:
Research Org.:
Brookhaven National Lab., Upton, NY (USA)
OSTI Identifier:
5528773
Application Number:
ON: DE86013777
Assignee:
Dept. of Energy TIC; ERA-11-005591; EDB-86-138279
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Resource Relation:
Other Information: Paper copy only, copy does not permit microfiche production
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACILLUS SUBTILIS; GENETIC ENGINEERING; PLASMIDS; DESIGN; STREPTOCOCCUS; RECOMBINANT DNA; BACILLUS; BACTERIA; CELL CONSTITUENTS; DNA; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; 550200* - Biochemistry; 550700 - Microbiology

Citation Formats

Lacks, S.A., and Balganesh, T.S. pLS101 plasmid vector. United States: N. p., 1985. Web.
Lacks, S.A., & Balganesh, T.S. pLS101 plasmid vector. United States.
Lacks, S.A., and Balganesh, T.S. Tue . "pLS101 plasmid vector". United States.
@article{osti_5528773,
title = {pLS101 plasmid vector},
author = {Lacks, S.A. and Balganesh, T.S.},
abstractNote = {Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb ma1M gene fragment ligated to a 4.4 Kb Tcr DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems. 5 figs., 2 tabs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1985},
month = {2}
}