pLS101 plasmid vector
Abstract
Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb ma1M gene fragment ligated to a 4.4 Kb Tcr DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems. 5 figs., 2 tabs.
- Inventors:
- Issue Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- OSTI Identifier:
- 5528773
- Application Number:
- ON: DE86013777
- Assignee:
- Dept. of Energy
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Resource Relation:
- Other Information: Paper copy only, copy does not permit microfiche production
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; BACILLUS SUBTILIS; GENETIC ENGINEERING; PLASMIDS; DESIGN; STREPTOCOCCUS; RECOMBINANT DNA; BACILLUS; BACTERIA; CELL CONSTITUENTS; DNA; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; 550200* - Biochemistry; 550700 - Microbiology
Citation Formats
Lacks, S A, and Balganesh, T S. pLS101 plasmid vector. United States: N. p., 1985.
Web.
Lacks, S A, & Balganesh, T S. pLS101 plasmid vector. United States.
Lacks, S A, and Balganesh, T S. Tue .
"pLS101 plasmid vector". United States.
@article{osti_5528773,
title = {pLS101 plasmid vector},
author = {Lacks, S A and Balganesh, T S},
abstractNote = {Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb ma1M gene fragment ligated to a 4.4 Kb Tcr DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems. 5 figs., 2 tabs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1985},
month = {2}
}