Continuous human cell lines and method of making same
Abstract
Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo(a)pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors. 2 tabs.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- OSTI Identifier:
- 5524665
- Application Number:
- ON: DE86013863
- Assignee:
- Dept. of Energy
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; CARCINOGEN SCREENING; DESIGN; CLONE CELLS; CELL PROLIFERATION; MUTAGEN SCREENING; BENZOPYRENE; BIOASSAY; CELL CULTURES; MAMMARY GLANDS; MAN; ANIMALS; AROMATICS; BODY; CONDENSED AROMATICS; GLANDS; HYDROCARBONS; MAMMALS; ORGANIC COMPOUNDS; ORGANS; PRIMATES; SCREENING; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology; 550300 - Cytology
Citation Formats
Stampfer, M R. Continuous human cell lines and method of making same. United States: N. p., 1985.
Web.
Stampfer, M R. Continuous human cell lines and method of making same. United States.
Stampfer, M R. Mon .
"Continuous human cell lines and method of making same". United States.
@article{osti_5524665,
title = {Continuous human cell lines and method of making same},
author = {Stampfer, M R},
abstractNote = {Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo(a)pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors. 2 tabs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jul 01 00:00:00 EDT 1985},
month = {Mon Jul 01 00:00:00 EDT 1985}
}