Rotor assembly and assay method
Abstract
A rotor assembly for carrying out an assay includes a rotor body which is rotatable about an axis of rotation, and has a central chamber and first, second, third, fourth, fifth, and sixth chambers which are in communication with and radiate from the central chamber. The rotor assembly further includes a shuttle which is movable through the central chamber and insertable into any of the chambers, the shuttle including a reaction cup carrying an immobilized antigen or an antibody for transport among the chambers. A method for carrying out an assay using the rotor assembly includes moving the reaction cup among the six chambers by passing the cup through the central chamber between centrifugation steps in order to perform the steps of: separating plasma from blood cells, binding plasma antibody or antigen, washing, drying, binding enzyme conjugate, reacting with enzyme substrate and optically comparing the resulting reaction product with unreacted enzyme substrate solution. The movement of the reaction cup can be provided by attaching a magnet to the reaction cup and supplying a moving magnetic field to the rotor. 34 figures.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 5440546
- Patent Number(s):
- 5242803
- Application Number:
- PPN: US 7-878799
- Assignee:
- Martin Marietta Energy Systems, Inc., Oak Ridge, TN (United States)
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 5 May 1992
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ANTIBODIES; BIOASSAY; ANTIGENS; BLOOD PLASMA; SEPARATION PROCESSES; ENZYMES; SEPARATION EQUIPMENT; DESIGN; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; MATERIALS; ORGANIC COMPOUNDS; PROTEINS; 550200* - Biochemistry
Citation Formats
Burtis, C A, Johnson, W F, and Walker, W A. Rotor assembly and assay method. United States: N. p., 1993.
Web.
Burtis, C A, Johnson, W F, & Walker, W A. Rotor assembly and assay method. United States.
Burtis, C A, Johnson, W F, and Walker, W A. Tue .
"Rotor assembly and assay method". United States.
@article{osti_5440546,
title = {Rotor assembly and assay method},
author = {Burtis, C A and Johnson, W F and Walker, W A},
abstractNote = {A rotor assembly for carrying out an assay includes a rotor body which is rotatable about an axis of rotation, and has a central chamber and first, second, third, fourth, fifth, and sixth chambers which are in communication with and radiate from the central chamber. The rotor assembly further includes a shuttle which is movable through the central chamber and insertable into any of the chambers, the shuttle including a reaction cup carrying an immobilized antigen or an antibody for transport among the chambers. A method for carrying out an assay using the rotor assembly includes moving the reaction cup among the six chambers by passing the cup through the central chamber between centrifugation steps in order to perform the steps of: separating plasma from blood cells, binding plasma antibody or antigen, washing, drying, binding enzyme conjugate, reacting with enzyme substrate and optically comparing the resulting reaction product with unreacted enzyme substrate solution. The movement of the reaction cup can be provided by attaching a magnet to the reaction cup and supplying a moving magnetic field to the rotor. 34 figures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {9}
}