Thermostable cellulase from a thermomonospora gene

Wilson, D B; Walker, L P; Zhang, S

Title: Thermostable cellulase from a thermomonospora gene

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Abstract

The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity. 3 figs.

Inventors:: Wilson, D B; Walker, L P; Zhang, S

Issue Date:: 1997-10-14

Research Org.:: Cornell Univ., Ithaca, NY (United States)

Sponsoring Org.:: USDOE, Washington, DC (United States)

OSTI Identifier:: 541742

Patent Number(s):: 5677151

Application Number:: PAN: 8-265,429

Assignee:: Cornell Research Foundation, Inc., Ithaca, NY (United States)

DOE Contract Number:: FG02-84ER13233

Resource Type:: Patent

Resource Relation:: Other Information: PBD: 14 Oct 1997

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; CELLULASE; GENES; AMINO ACID SEQUENCE; NUCLEOTIDES; GENE RECOMBINATION; ENZYME ACTIVITY; ENZYMATIC HYDROLYSIS

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                    Wilson, D B, Walker, L P, and Zhang, S. Thermostable cellulase from a thermomonospora gene.  United States: N. p., 1997. 
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                    Wilson, D B, Walker, L P, & Zhang, S. Thermostable cellulase from a thermomonospora gene.  United States.

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                    Wilson, D B, Walker, L P, and Zhang, S. Tue .  
        "Thermostable cellulase from a thermomonospora gene".  United States.

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@article{osti_541742,

  title        = {Thermostable cellulase from a thermomonospora gene},

  author       = {Wilson, D B and Walker, L P and Zhang, S},

  abstractNote = {The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity. 3 figs.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1997},

  month        = {10}

}

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The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity.
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