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Title: Improved method and apparatus for electrostatically sorting biological cells. [DOE patent application]

Abstract

An improved method of sorting biological cells in a conventional cell sorter apparatus includes generating a fluid jet containing cells to be sorted, measuring the distance between the centers of adjacent droplets in a zone thereof defined at the point where the fluid jet separates into descrete droplets, setting the distance between the center of a droplet in said separation zone and the position along said fluid jet at which the cell is optically sensed for specific characteristics to be an integral multiple of said center-to-center distance, and disabling a charger from electrically charging a specific droplet if a cell is detected by the optical sensor in a position wherein it will be in the neck area between droplets during droplet formation rather than within a predetermined distance from the droplet center.

Inventors:
OSTI Identifier:
5415113
Assignee:
TIC; ERA-05-024701; EDB-80-067995
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; ANIMAL CELLS; ELECTROSTATIC SEPARATION; CELL FLOW SYSTEMS; DESIGN; CYTOLOGICAL TECHNIQUES; SORTING; DIAGRAMS; SEPARATION PROCESSES; 550300* - Cytology; 440300 - Miscellaneous Instruments- (-1989)

Citation Formats

Merrill, J T. Improved method and apparatus for electrostatically sorting biological cells. [DOE patent application]. United States: N. p., Web.
Merrill, J T. Improved method and apparatus for electrostatically sorting biological cells. [DOE patent application]. United States.
Merrill, J T. . "Improved method and apparatus for electrostatically sorting biological cells. [DOE patent application]". United States.
@article{osti_5415113,
title = {Improved method and apparatus for electrostatically sorting biological cells. [DOE patent application]},
author = {Merrill, J T},
abstractNote = {An improved method of sorting biological cells in a conventional cell sorter apparatus includes generating a fluid jet containing cells to be sorted, measuring the distance between the centers of adjacent droplets in a zone thereof defined at the point where the fluid jet separates into descrete droplets, setting the distance between the center of a droplet in said separation zone and the position along said fluid jet at which the cell is optically sensed for specific characteristics to be an integral multiple of said center-to-center distance, and disabling a charger from electrically charging a specific droplet if a cell is detected by the optical sensor in a position wherein it will be in the neck area between droplets during droplet formation rather than within a predetermined distance from the droplet center.},
doi = {},
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place = {United States},
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}