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Title: Apparatus for reading two-dimensional electrophoretograms containing. beta. -ray-emitting labeled compounds

Abstract

An apparatus and method for electronically reading planar two-dimensional ..beta..-ray emitter-labeled gel electrophoretograms. A single, flat rectangular multiwire proportional chamber is placed in close proximity to the gel and the assembly placed in an intense uniform magnetic field disposed in a perpendicular manner to the rectangular face of the proportional chamber. Beta rays emitted in the direction of the proportional chamber are caused to execute helical motions which substantially preserve knowledge the coordinates of their origin in the gel. Perpendicularly oriented, parallel wire, parallel plane cathodes electronically sense the location of the ..beta..-rays from ionization generated thereby in a detection gas coupled with an electron avalanche effect resulting from the action of a parallel wire anode located therebetween. A scintillator permits the present apparatus to be rendered insensitive when signals are generated from cosmic rays incident on the proportional chamber. Resolution for concentrations of radioactive compounds in the gel exceeds 700-..mu..m. The apparatus and method of the present invention represent a significant improvement over conventional autoradiographic techniques in dynamic range, linearity and sensitivity of data collection. A concentration and position map for gel electrophoretograms having significant concentrations of labeled compounds and/or highly radioactive labeling nuclides can generally be obtained inmore » less than one hour.« less

Inventors:
; ;
Issue Date:
Research Org.:
Los Alamos National Lab., NM (USA)
OSTI Identifier:
5387529
Application Number:
ON: DE86013865
Assignee:
Dept. of Energy TIC; EDB-86-145604
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; ELECTROPHORESIS; LABELLED COMPOUNDS; MULTIWIRE PROPORTIONAL CHAMBERS; USES; ANTICOINCIDENCE; BETA DECAY RADIOISOTOPES; SCINTILLATION COUNTERS; ISOTOPES; MEASURING INSTRUMENTS; PROPORTIONAL COUNTERS; RADIATION DETECTORS; RADIOISOTOPES; 440101* - Radiation Instrumentation- General Detectors or Monitors & Radiometric Instruments

Citation Formats

Anderson, H.L., Kinnison, W.W., and Lillberg, J.W. Apparatus for reading two-dimensional electrophoretograms containing. beta. -ray-emitting labeled compounds. United States: N. p., 1985. Web.
Anderson, H.L., Kinnison, W.W., & Lillberg, J.W. Apparatus for reading two-dimensional electrophoretograms containing. beta. -ray-emitting labeled compounds. United States.
Anderson, H.L., Kinnison, W.W., and Lillberg, J.W. Tue . "Apparatus for reading two-dimensional electrophoretograms containing. beta. -ray-emitting labeled compounds". United States.
@article{osti_5387529,
title = {Apparatus for reading two-dimensional electrophoretograms containing. beta. -ray-emitting labeled compounds},
author = {Anderson, H.L. and Kinnison, W.W. and Lillberg, J.W.},
abstractNote = {An apparatus and method for electronically reading planar two-dimensional ..beta..-ray emitter-labeled gel electrophoretograms. A single, flat rectangular multiwire proportional chamber is placed in close proximity to the gel and the assembly placed in an intense uniform magnetic field disposed in a perpendicular manner to the rectangular face of the proportional chamber. Beta rays emitted in the direction of the proportional chamber are caused to execute helical motions which substantially preserve knowledge the coordinates of their origin in the gel. Perpendicularly oriented, parallel wire, parallel plane cathodes electronically sense the location of the ..beta..-rays from ionization generated thereby in a detection gas coupled with an electron avalanche effect resulting from the action of a parallel wire anode located therebetween. A scintillator permits the present apparatus to be rendered insensitive when signals are generated from cosmic rays incident on the proportional chamber. Resolution for concentrations of radioactive compounds in the gel exceeds 700-..mu..m. The apparatus and method of the present invention represent a significant improvement over conventional autoradiographic techniques in dynamic range, linearity and sensitivity of data collection. A concentration and position map for gel electrophoretograms having significant concentrations of labeled compounds and/or highly radioactive labeling nuclides can generally be obtained in less than one hour.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1985},
month = {4}
}