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Title: Improved monoclonal antibodies to halodeoxyuridine

Abstract

The development, method of production, characterization and methods of use of two hybridomas, CIdU-1 (ATCC Accession No. HB-8321) and CIdU-2 (ATCC Accession No. HB-8320), are described. These secrete IgG/sub 1/(K) immunoglobulins that react with halodeoxyuridine (HdU or halodU) such as bromo, chloro, fluoro and iodo deoxyuridine (BrdU, CldU, FdU and IdU), whether these are free in solution or incorporated into single stranded DNA in whole cells. The antibodies do not react with naturally occurring free nucleic acids or with deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) polymers. These antibodies are suitable for use in enzyme immunoassays for free CldU, FdU, IdU and BrdU and for detecting cells with these nucleotides incorporated into them. The monoclonal antibodies are useful in the detection of the sensitivity of tumor cells to specific chemotherapeutic agents, in the measurement of the rate of cellular DNA synthesis, in the measurement of the rate of proliferation of normal and malignant cells and in the detection of HPRT deficiency in cells. 1 tab.

Inventors:
; ; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab., CA (USA)
OSTI Identifier:
5313860
Application Number:
ON: DE85017764
Assignee:
Dept. of Energy EDB-85-149536
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; BUDR; MONOCLONAL ANTIBODIES; FUDR; IODODEOXYURIDINE; DIAGNOSTIC USES; CELL FLOW SYSTEMS; CHO CELLS; DNA REPAIR; ENZYME IMMUNOASSAY; HYBRIDOMAS; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; ANIMAL CELLS; ANTI-INFECTIVE AGENTS; ANTIBODIES; ANTIMETABOLITES; ANTIMICROBIAL AGENTS; AZINES; BIOASSAY; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BROMOURACILS; DRUGS; ENZYMES; FLUOROURACILS; GLYCOSYL TRANSFERASES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; IODOURACILS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC BROMINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC FLUORINE COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC IODINE COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PENTOSYL TRANSFERASES; PYRIMIDINES; RADIOSENSITIZERS; RECOVERY; REPAIR; RIBOSIDES; TRANSFERASES; URACILS; USES; 550600* - Medicine; 550900 - Pathology

Citation Formats

Vanderlaan, M., Dolbeare, F.A., Gray, J.W., and Thomas, C.B. Improved monoclonal antibodies to halodeoxyuridine. United States: N. p., 1983. Web.
Vanderlaan, M., Dolbeare, F.A., Gray, J.W., & Thomas, C.B. Improved monoclonal antibodies to halodeoxyuridine. United States.
Vanderlaan, M., Dolbeare, F.A., Gray, J.W., and Thomas, C.B. Tue . "Improved monoclonal antibodies to halodeoxyuridine". United States.
@article{osti_5313860,
title = {Improved monoclonal antibodies to halodeoxyuridine},
author = {Vanderlaan, M. and Dolbeare, F.A. and Gray, J.W. and Thomas, C.B.},
abstractNote = {The development, method of production, characterization and methods of use of two hybridomas, CIdU-1 (ATCC Accession No. HB-8321) and CIdU-2 (ATCC Accession No. HB-8320), are described. These secrete IgG/sub 1/(K) immunoglobulins that react with halodeoxyuridine (HdU or halodU) such as bromo, chloro, fluoro and iodo deoxyuridine (BrdU, CldU, FdU and IdU), whether these are free in solution or incorporated into single stranded DNA in whole cells. The antibodies do not react with naturally occurring free nucleic acids or with deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) polymers. These antibodies are suitable for use in enzyme immunoassays for free CldU, FdU, IdU and BrdU and for detecting cells with these nucleotides incorporated into them. The monoclonal antibodies are useful in the detection of the sensitivity of tumor cells to specific chemotherapeutic agents, in the measurement of the rate of cellular DNA synthesis, in the measurement of the rate of proliferation of normal and malignant cells and in the detection of HPRT deficiency in cells. 1 tab.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1983},
month = {10}
}