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Title: Method to transform algae, materials therefor, and products produced thereby

Abstract

Disclosed is a method to transform chlorophyll C-containing algae. The method includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further, specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae. 2 figs.

Inventors:
; ;
Issue Date:
Research Org.:
Midwest Research Institute
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
527757
Patent Number(s):
5,661,017
Application Number:
PAN: 8-404,732
Assignee:
PTO; SCA: 550200; PA: EDB-97:127301; SN: 97001843779
DOE Contract Number:  
AC36-83CH10093
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 26 Aug 1997
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ALGAE; GENETIC ENGINEERING; RECOMBINANT DNA; CHLOROPHYLL; NUCLEIC ACIDS

Citation Formats

Dunahay, T.G., Roessler, P.G., and Jarvis, E.E. Method to transform algae, materials therefor, and products produced thereby. United States: N. p., 1997. Web.
Dunahay, T.G., Roessler, P.G., & Jarvis, E.E. Method to transform algae, materials therefor, and products produced thereby. United States.
Dunahay, T.G., Roessler, P.G., and Jarvis, E.E. Tue . "Method to transform algae, materials therefor, and products produced thereby". United States.
@article{osti_527757,
title = {Method to transform algae, materials therefor, and products produced thereby},
author = {Dunahay, T.G. and Roessler, P.G. and Jarvis, E.E.},
abstractNote = {Disclosed is a method to transform chlorophyll C-containing algae. The method includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further, specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae. 2 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {8}
}