Method to transform algae, materials therefor, and products produced thereby
Abstract
Disclosed is a method to transform chlorophyll C-containing algae. The method includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further, specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae. 2 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 527757
- Patent Number(s):
- 5661017
- Application Number:
- PAN: 8-404,732
- Assignee:
- PTO; SCA: 550200; PA: EDB-97:127301; SN: 97001843779
- DOE Contract Number:
- AC36-83CH10093
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 26 Aug 1997
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; ALGAE; GENETIC ENGINEERING; RECOMBINANT DNA; CHLOROPHYLL; NUCLEIC ACIDS
Citation Formats
Dunahay, T G, Roessler, P G, and Jarvis, E E. Method to transform algae, materials therefor, and products produced thereby. United States: N. p., 1997.
Web.
Dunahay, T G, Roessler, P G, & Jarvis, E E. Method to transform algae, materials therefor, and products produced thereby. United States.
Dunahay, T G, Roessler, P G, and Jarvis, E E. Tue .
"Method to transform algae, materials therefor, and products produced thereby". United States.
@article{osti_527757,
title = {Method to transform algae, materials therefor, and products produced thereby},
author = {Dunahay, T G and Roessler, P G and Jarvis, E E},
abstractNote = {Disclosed is a method to transform chlorophyll C-containing algae. The method includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further, specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae. 2 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {8}
}