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Title: Liquid chromatography/Fourier transform IR spectrometry interface flow cell

Abstract

A zero dead volume (ZDV) microbore high performance liquid chromatography (..mu.. HPLC)/Fourier transform infrared (FTIR) interface flow cell includes an IR transparent crystal having a small diameter bore therein through which a sample liquid is passed. The interface flow cell further includes a metal holder in combination with a pair of inner, compressible seals for directly coupling the thus configured spectrometric flow cell to the outlet of a ..mu.. HPLC column end fitting to minimize the transfer volume of the effluents exiting the ..mu.. HPLC column which exhibit excellent flow characteristics due to the essentially unencumbered, open-flow design. The IR beam passes transverse to the sample flow through the circular bore within the IR transparent crystal, which is preferably comprised of potassium bromide (KBr) or calcium fluoride (CaF/sub 2/), so as to minimize interference patterns and vignetting encountered in conventional parallel-plate IR cells. The long IR beam pathlength and lensing effect of the circular cross-section of the sample volume in combination with the refractive index differences between the solvent and the transparent crystal serve to focus the IR beam in enhancing sample detection sensitivity by an order of magnitude.

Inventors:
;
Issue Date:
Research Org.:
Virginia Polytechnic Inst. and State Univ., Blacksburg (USA)
OSTI Identifier:
5204823
Application Number:
ON: DE85017749
Assignee:
Dept. of Energy NETL; ERA-10-049600; EDB-85-157997
DOE Contract Number:  
FG22-81PC40799
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; LIQUID COLUMN CHROMATOGRAPHY; FOURIER TRANSFORM SPECTROMETERS; CALCIUM FLUORIDES; POTASSIUM BROMIDES; ALKALI METAL COMPOUNDS; ALKALINE EARTH METAL COMPOUNDS; BROMIDES; BROMINE COMPOUNDS; CALCIUM COMPOUNDS; CALCIUM HALIDES; CHROMATOGRAPHY; FLUORIDES; FLUORINE COMPOUNDS; HALIDES; HALOGEN COMPOUNDS; MEASURING INSTRUMENTS; POTASSIUM COMPOUNDS; SEPARATION PROCESSES; SPECTROMETERS; 440300* - Miscellaneous Instruments- (-1989)

Citation Formats

Johnson, C.C., and Taylor, L.T. Liquid chromatography/Fourier transform IR spectrometry interface flow cell. United States: N. p., 1985. Web.
Johnson, C.C., & Taylor, L.T. Liquid chromatography/Fourier transform IR spectrometry interface flow cell. United States.
Johnson, C.C., and Taylor, L.T. Fri . "Liquid chromatography/Fourier transform IR spectrometry interface flow cell". United States.
@article{osti_5204823,
title = {Liquid chromatography/Fourier transform IR spectrometry interface flow cell},
author = {Johnson, C.C. and Taylor, L.T.},
abstractNote = {A zero dead volume (ZDV) microbore high performance liquid chromatography (..mu.. HPLC)/Fourier transform infrared (FTIR) interface flow cell includes an IR transparent crystal having a small diameter bore therein through which a sample liquid is passed. The interface flow cell further includes a metal holder in combination with a pair of inner, compressible seals for directly coupling the thus configured spectrometric flow cell to the outlet of a ..mu.. HPLC column end fitting to minimize the transfer volume of the effluents exiting the ..mu.. HPLC column which exhibit excellent flow characteristics due to the essentially unencumbered, open-flow design. The IR beam passes transverse to the sample flow through the circular bore within the IR transparent crystal, which is preferably comprised of potassium bromide (KBr) or calcium fluoride (CaF/sub 2/), so as to minimize interference patterns and vignetting encountered in conventional parallel-plate IR cells. The long IR beam pathlength and lensing effect of the circular cross-section of the sample volume in combination with the refractive index differences between the solvent and the transparent crystal serve to focus the IR beam in enhancing sample detection sensitivity by an order of magnitude.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1985},
month = {1}
}