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Title: Fluorometric method of quantitative cell mutagenesis

Abstract

A method for assaying a cell culture for mutagenesis is described. A cell culture is stained first with a histochemical stain, and then a fluorescent stain. Normal cells in the culture are stained by both the histochemical and fluorescent stains, while abnormal cells are stained only by the fluorescent stain. The two stains are chosen so that the histochemical stain absorbs the wavelengths that the fluorescent stain emits. After the counterstained culture is subjected to exciting light, the fluorescence from the abnormal cells is detected.

Inventors:
Issue Date:
OSTI Identifier:
5177161
Assignee:
Dept. of Energy EDB-82-131237
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL CULTURES; MUTAGENESIS; HISTOLOGICAL TECHNIQUES; ACCURACY; BANDING TECHNIQUES; BIOASSAY; FLUORESCENCE; HISTOLOGY; MEASURING METHODS; QUANTITATIVE CHEMICAL ANALYSIS; STAINS; CHEMICAL ANALYSIS; LUMINESCENCE; 550300* - Cytology; 550400 - Genetics

Citation Formats

Dolbeare, F.A. Fluorometric method of quantitative cell mutagenesis. United States: N. p., 1980. Web.
Dolbeare, F.A. Fluorometric method of quantitative cell mutagenesis. United States.
Dolbeare, F.A. Fri . "Fluorometric method of quantitative cell mutagenesis". United States.
@article{osti_5177161,
title = {Fluorometric method of quantitative cell mutagenesis},
author = {Dolbeare, F.A.},
abstractNote = {A method for assaying a cell culture for mutagenesis is described. A cell culture is stained first with a histochemical stain, and then a fluorescent stain. Normal cells in the culture are stained by both the histochemical and fluorescent stains, while abnormal cells are stained only by the fluorescent stain. The two stains are chosen so that the histochemical stain absorbs the wavelengths that the fluorescent stain emits. After the counterstained culture is subjected to exciting light, the fluorescence from the abnormal cells is detected.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1980},
month = {12}
}