Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068
Abstract
A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068) is presented. The cellulase is water soluble, possesses both C[sub 1] and C[sub x] types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83 C at pH's from about 2 to about 9, and in inactivation temperature of about 110 C at pH's from about 2 to about 9. 7 figures.
- Inventors:
- Issue Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States); Midwest Research Institute, Kansas City, MO (United States)
- OSTI Identifier:
- 5147550
- Patent Number(s):
- 5275944
- Application Number:
- PPN: US 7-826089
- Assignee:
- Midwest Research Inst., Kansas City, MO ()
- DOE Contract Number:
- AC02-83CH10093
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 27 Jan 1992
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; BACTERIA; BIOCHEMISTRY; ENDONUCLEASES; ENZYME ACTIVITY; CHEMICAL PROPERTIES; CHEMISTRY; DNA-ASE; ENZYMES; ESTERASES; HYDROLASES; MICROORGANISMS; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; PROTEINS; 550200* - Biochemistry
Citation Formats
Himmel, M E, Adney, W S, Tucker, M P, and Grohmann, K. Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068. United States: N. p., 1994.
Web.
Himmel, M E, Adney, W S, Tucker, M P, & Grohmann, K. Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068. United States.
Himmel, M E, Adney, W S, Tucker, M P, and Grohmann, K. Tue .
"Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068". United States.
@article{osti_5147550,
title = {Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068},
author = {Himmel, M E and Adney, W S and Tucker, M P and Grohmann, K},
abstractNote = {A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068) is presented. The cellulase is water soluble, possesses both C[sub 1] and C[sub x] types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83 C at pH's from about 2 to about 9, and in inactivation temperature of about 110 C at pH's from about 2 to about 9. 7 figures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 04 00:00:00 EST 1994},
month = {Tue Jan 04 00:00:00 EST 1994}
}
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