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Title: Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068

Abstract

A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068) is presented. The cellulase is water soluble, possesses both C[sub 1] and C[sub x] types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83 C at pH's from about 2 to about 9, and in inactivation temperature of about 110 C at pH's from about 2 to about 9. 7 figures.

Inventors:
; ; ;
Issue Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Midwest Research Institute
OSTI Identifier:
5147550
Patent Number(s):
5275944 A
Application Number:
PPN: US 7-826089
Assignee:
Midwest Research Inst., Kansas City, MO () NREL; EDB-94-065663
DOE Contract Number:  
AC02-83CH10093
Resource Type:
Patent
Resource Relation:
Patent File Date: 27 Jan 1992
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACTERIA; BIOCHEMISTRY; ENDONUCLEASES; ENZYME ACTIVITY; CHEMICAL PROPERTIES; CHEMISTRY; DNA-ASE; ENZYMES; ESTERASES; HYDROLASES; MICROORGANISMS; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; PROTEINS; 550200* - Biochemistry

Citation Formats

Himmel, M.E., Adney, W.S., Tucker, M.P., and Grohmann, K. Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068. United States: N. p., 1994. Web.
Himmel, M.E., Adney, W.S., Tucker, M.P., & Grohmann, K. Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068. United States.
Himmel, M.E., Adney, W.S., Tucker, M.P., and Grohmann, K. Tue . "Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068". United States.
@article{osti_5147550,
title = {Thermostable purified endoglucanase from Acidothermus cellulolyticus ATCC 43068},
author = {Himmel, M.E. and Adney, W.S. and Tucker, M.P. and Grohmann, K.},
abstractNote = {A purified low molecular weight cellulase endoglucanase I having a molecular weight of between about 57,420 to about 74,580 daltons from Acidothermus cellulolyticus (ATCC 43068) is presented. The cellulase is water soluble, possesses both C[sub 1] and C[sub x] types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 83 C at pH's from about 2 to about 9, and in inactivation temperature of about 110 C at pH's from about 2 to about 9. 7 figures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {1}
}