Method for extending the useful shelf-life of refrigerated red blood cells by flushing with inert gas
A method is disclosed using oxygen removal for extending the useful shelf-life of refrigerated red blood cells. A cost-effective, 4 C storage procedure that preserves red cell quality and prolongs post-transfusion in vivo survival is described. Preservation of adenosine triphosphate levels and reduction in hemolysis and in membrane vesicle production of red blood cells stored at 4 C for prolonged periods of time is achieved by removing oxygen from the red blood cells at the time of storage; in particular, by flushing with an inert gas. Adenosine triphosphate levels of the stored red blood cells are boosted in some samples by addition of ammonium phosphate. 4 figs.
- Issue Date:
- OSTI Identifier:
- 504949
- Assignee:
- Univ. of California, Los Alamos, NM (United States) PTO; SCA: 550500; PA: EDB-97:101409; SN: 97001815607
- Patent Number(s):
- US 5,624,794/A/
- Application Number:
- PAN: 8-473,675
- Contract Number:
- W-7405-ENG-36
- Resource Relation:
- Other Information: PBD: 29 Apr 1997
- Research Org:
- University of California
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; ERYTHROCYTES; STORAGE LIFE; OXYGEN; REMOVAL; TRANSFUSIONS; ATP; CELL MEMBRANES