Method for extending the useful shelf-life of refrigerated red blood cells by flushing with inert gas
Abstract
A method is disclosed using oxygen removal for extending the useful shelf-life of refrigerated red blood cells. A cost-effective, 4 C storage procedure that preserves red cell quality and prolongs post-transfusion in vivo survival is described. Preservation of adenosine triphosphate levels and reduction in hemolysis and in membrane vesicle production of red blood cells stored at 4 C for prolonged periods of time is achieved by removing oxygen from the red blood cells at the time of storage; in particular, by flushing with an inert gas. Adenosine triphosphate levels of the stored red blood cells are boosted in some samples by addition of ammonium phosphate. 4 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 504949
- Patent Number(s):
- 5624794
- Application Number:
- PAN: 8-473,675
- Assignee:
- Univ. of California, Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 29 Apr 1997
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; ERYTHROCYTES; STORAGE LIFE; OXYGEN; REMOVAL; TRANSFUSIONS; ATP; CELL MEMBRANES
Citation Formats
Bitensky, M W, and Yoshida, Tatsuro. Method for extending the useful shelf-life of refrigerated red blood cells by flushing with inert gas. United States: N. p., 1997.
Web.
Bitensky, M W, & Yoshida, Tatsuro. Method for extending the useful shelf-life of refrigerated red blood cells by flushing with inert gas. United States.
Bitensky, M W, and Yoshida, Tatsuro. Tue .
"Method for extending the useful shelf-life of refrigerated red blood cells by flushing with inert gas". United States.
@article{osti_504949,
title = {Method for extending the useful shelf-life of refrigerated red blood cells by flushing with inert gas},
author = {Bitensky, M W and Yoshida, Tatsuro},
abstractNote = {A method is disclosed using oxygen removal for extending the useful shelf-life of refrigerated red blood cells. A cost-effective, 4 C storage procedure that preserves red cell quality and prolongs post-transfusion in vivo survival is described. Preservation of adenosine triphosphate levels and reduction in hemolysis and in membrane vesicle production of red blood cells stored at 4 C for prolonged periods of time is achieved by removing oxygen from the red blood cells at the time of storage; in particular, by flushing with an inert gas. Adenosine triphosphate levels of the stored red blood cells are boosted in some samples by addition of ammonium phosphate. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {4}
}
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