Normalized cDNA libraries
Abstract
This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Columbia Univ., New York, NY (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 489094
- Patent Number(s):
- 5637685
- Application Number:
- PAN: 8-465,811
- Assignee:
- Columbia Univ., New York, NY (United States)
- DOE Contract Number:
- FG02-91ER61233
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 10 Jun 1997
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; DNA SEQUENCING; DNA; PHENOTYPE; GENES
Citation Formats
Soares, M B, and Efstratiadis, A. Normalized cDNA libraries. United States: N. p., 1997.
Web.
Soares, M B, & Efstratiadis, A. Normalized cDNA libraries. United States.
Soares, M B, and Efstratiadis, A. Tue .
"Normalized cDNA libraries". United States.
@article{osti_489094,
title = {Normalized cDNA libraries},
author = {Soares, M B and Efstratiadis, A},
abstractNote = {This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {6}
}