Normalized cDNA libraries

Title: Normalized cDNA libraries

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Abstract

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

Inventors:: Soares, M B; Efstratiadis, A

Issue Date:: 1997-06-10

Research Org.:: Columbia Univ., New York, NY (United States)

Sponsoring Org.:: USDOE, Washington, DC (United States)

OSTI Identifier:: 489094

Patent Number(s):: 5637685

Application Number:: PAN: 8-465,811

Assignee:: Columbia Univ., New York, NY (United States)

DOE Contract Number:: FG02-91ER61233

Resource Type:: Patent

Resource Relation:: Other Information: PBD: 10 Jun 1997

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; DNA SEQUENCING; DNA; PHENOTYPE; GENES

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                    Soares, M B, and Efstratiadis, A. Normalized cDNA libraries.  United States: N. p., 1997. 
        Web.

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                    Soares, M B, & Efstratiadis, A. Normalized cDNA libraries.  United States.

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                    Soares, M B, and Efstratiadis, A. Tue .  
        "Normalized cDNA libraries".  United States.

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@article{osti_489094,

  title        = {Normalized cDNA libraries},

  author       = {Soares, M B and Efstratiadis, A},

  abstractNote = {This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1997},

  month        = {6}

}

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