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Title: Multiplexed capillary electrophoresis system

Abstract

The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Iowa State Univ., Ames, IA (United States)
OSTI Identifier:
415746
Patent Number(s):
5,582,705
Application Number:
PAN: 8-444,565
Assignee:
Iowa State Univ. Research Foundation, Inc., Ames, IA (United States)
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 10 Dec 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; EQUIPMENT; DESIGN; NUCLEOTIDES; ELECTROPHORESIS; MOLECULAR BIOLOGY

Citation Formats

Yeung, E S, Chang, H T, Fung, E N, Li, Q, and Lu, X. Multiplexed capillary electrophoresis system. United States: N. p., 1996. Web.
Yeung, E S, Chang, H T, Fung, E N, Li, Q, & Lu, X. Multiplexed capillary electrophoresis system. United States.
Yeung, E S, Chang, H T, Fung, E N, Li, Q, and Lu, X. Tue . "Multiplexed capillary electrophoresis system". United States.
@article{osti_415746,
title = {Multiplexed capillary electrophoresis system},
author = {Yeung, E S and Chang, H T and Fung, E N and Li, Q and Lu, X},
abstractNote = {The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {12}
}