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Title: Rapid purification of circular DNA by triplex-mediated affinity capture

A single-step capture of a target supercoiled double-stranded DNA molecule is accomplished by forming a local triple-helix among two strands of the supercoiled circular DNA and an oligonucleotide probe. The oligonucleotide is bound to an immobilizing support which facilitates the immobilization and purification of target DNA molecules. Non-target DNA molecules and other contaminating cellular material are easily removed by washing. The triple-helical structure is destabilized by raising the pH, leaving purified target DNA in the supernatant and reusable affinity capture oligonucleotide secured to the immobilizing support. 3 figs.
Inventors:
;
Issue Date:
OSTI Identifier:
415738
Assignee:
PTO; SCA: 550200; PA: EDB-97:016814; SN: 97001714576
Patent Number(s):
US 5,591,841/A/
Application Number:
PAN: 8-317,102
Contract Number:
FG02-90ER61026
Resource Relation:
Other Information: PBD: 7 Jan 1997
Research Org:
University of Wisconsin
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA; PURIFICATION; MOLECULAR STRUCTURE; MOLECULAR BIOLOGY; HELICAL CONFIGURATION