Triple helix purification and sequencing

Wang, R; Smith, L M; Tong, X E

Title: Triple helix purification and sequencing

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Abstract

Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis. 4 figures.

Inventors:: Wang, R; Smith, L M; Tong, X E

Issue Date:: 1995-03-28

Research Org.:: Univ. of Wisconsin, Madison, WI (United States)

OSTI Identifier:: 35065

Patent Number(s):: 5401632

Application Number:: PAN: 7-915,245

Assignee:: Wisconsin Alumni Research Foundation, Madison, WI (United States)

DOE Contract Number:: FG02-90ER61026

Resource Type:: Patent

Resource Relation:: Other Information: PBD: 28 Mar 1995

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; DNA; PURIFICATION; OLIGONUCLEOTIDES; DNA HYBRIDIZATION; HELICAL CONFIGURATION; MOLECULAR STRUCTURE

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                    Wang, R, Smith, L M, and Tong, X E. Triple helix purification and sequencing.  United States: N. p., 1995. 
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                    Wang, R, Smith, L M, & Tong, X E. Triple helix purification and sequencing.  United States.

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                    Wang, R, Smith, L M, and Tong, X E. Tue .  
        "Triple helix purification and sequencing".  United States.

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@article{osti_35065,

  title        = {Triple helix purification and sequencing},

  author       = {Wang, R and Smith, L M and Tong, X E},

  abstractNote = {Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis. 4 figures.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1995},

  month        = {3}

}

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