Triple helix purification and sequencing

Title: Triple helix purification and sequencing

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Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis. 4 figures.

Inventors:: Wang, R.; Smith, L.M.; Tong, X.E.

Issue Date:: 1995-03-28

OSTI Identifier:: 35065

Assignee:: Wisconsin Alumni Research Foundation, Madison, WI (United States) PTO; SCA: 550200; PA: EDB-95:065935; SN: 95001365540

Patent Number(s):: US 5,401,632/A/

Application Number:: PAN: 7-915,245

Contract Number:: FG02-90ER61026

Resource Relation:: Other Information: PBD: 28 Mar 1995

Research Org:: University of Wisconsin

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; DNA; PURIFICATION; OLIGONUCLEOTIDES; DNA HYBRIDIZATION; HELICAL CONFIGURATION; MOLECULAR STRUCTURE

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