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Title: Triple helix purification and sequencing

Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis. 4 figures.
Inventors:
; ;
Issue Date:
OSTI Identifier:
35065
Assignee:
Wisconsin Alumni Research Foundation, Madison, WI (United States) PTO; SCA: 550200; PA: EDB-95:065935; SN: 95001365540
Patent Number(s):
US 5,401,632/A/
Application Number:
PAN: 7-915,245
Contract Number:
FG02-90ER61026
Resource Relation:
Other Information: PBD: 28 Mar 1995
Research Org:
University of Wisconsin
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; DNA; PURIFICATION; OLIGONUCLEOTIDES; DNA HYBRIDIZATION; HELICAL CONFIGURATION; MOLECULAR STRUCTURE

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