Triple helix purification and sequencing

Title: Triple helix purification and sequencing

Full Record
Other Related Research

Abstract

Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis. 4 figures.

Inventors:: Wang, R; Smith, L M; Tong, X E

Issue Date:: 1995-03-28

Research Org.:: University of Wisconsin

OSTI Identifier:: 35065

Patent Number(s):: 5,401,632

Application Number:: PAN: 7-915,245

Assignee:: Wisconsin Alumni Research Foundation, Madison, WI (United States)

DOE Contract Number:: FG02-90ER61026

Resource Type:: Patent

Resource Relation:: Other Information: PBD: 28 Mar 1995

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; DNA; PURIFICATION; OLIGONUCLEOTIDES; DNA HYBRIDIZATION; HELICAL CONFIGURATION; MOLECULAR STRUCTURE

Citation Formats


                    Wang, R, Smith, L M, and Tong, X E. Triple helix purification and sequencing.  United States: N. p., 1995. 
        Web.

Copy to clipboard


                    Wang, R, Smith, L M, & Tong, X E. Triple helix purification and sequencing.  United States.

Copy to clipboard


                    Wang, R, Smith, L M, and Tong, X E. Tue .  
        "Triple helix purification and sequencing".  United States.

Copy to clipboard


                    
@article{osti_35065,

  title        = {Triple helix purification and sequencing},

  author       = {Wang, R and Smith, L M and Tong, X E},

  abstractNote = {Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis. 4 figures.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1995},

  month        = {3}

}

Copy to clipboard

Patent:

Search for the full text at the U.S. Patent and Trademark Office

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE Patents and OSTI.GOV collections:

Triple helix purification and sequencing

Patent Wang, Renfeng [Dublin, CA]; Smith, Lloyd M [Madison, WI]; Tong, Xinchun E [Madison, WI]

Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can bemore »« less
Full Text Available
Distillation sequence for the purification and recovery of hydrocarbons

Patent Reyneke, Rian [Katy, TX]; Foral, Michael [Aurora, IL]; Papadopoulos, Christos G [Naperville, IL]; ...

This invention is an improved distillation sequence for the separation and purification of ethylene from a cracked gas. A hydrocarbon feed enters a C2 distributor column. The top of the C2 distributor column is thermally coupled to an ethylene distributor column, and the bottoms liquid of a C2 distributor column feeds a deethanizer column. The C2 distributor column utilizes a conventional reboiler. The top of the ethylene distributor is thermally coupled with a demethanizer column, and the bottoms liquid of the ethylene distributor feeds a C2 splitter column. The ethylene distributor column utilizes a conventional reboiler. The deethanizer and C2more »« less
Full Text Available
Helix coupling

Patent Ginell, W S

A coupling for connecting helix members in series, which consists of a pair of U-shaped elements, one of which is attached to each helix end with the U sections of the elements interlocked. The coupling is particularly beneficial for interconnecting helical Nitinol elements utilized in thermal actuators or engines. Each coupling half is attached to the associated helix at two points, thereby providing axial load while being easily removed from the helix, and reusable.
Shielded helix traveling wave cathode ray tube deflection structure

Patent Norris, N J; Hudson, C L

Various embodiments of a helical coil deflection structure of a CRT are described and illustrated which provide shielding between adjacent turns of the coil on either three or four sides of each turn in the coil. Threaded members formed with either male or female threads and having the same pitch as the deflection coil are utilized for shielding the deflection coil with each turn of the helical coil placed between adjacent threads which act to shield each coil turn from adjacent turns and to confine the field generated by the coil to prevent or inhibit cross-coupling between adjacent turns ofmore »« less
Helix coupling

Patent Ginell, W S

A coupling for connecting helix members in series, which consists of a pair of U-shaped elements, one of which is attached to each helix end with the "U" sections of the elements interlocked. The coupling is particularly beneficial for interconnecting helical Nitinol elements utilized in thermal actuators or engines. Each coupling half is attached to the associated helix at two points, thereby providing axial load while being easily removed from the helix, and reusable.

Similar Records