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Title: Method for rapid base sequencing in DNA and RNA with two base labeling

Abstract

A method is described for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand. 4 figures.

Inventors:
; ; ; ; ; ;
Issue Date:
Research Org.:
University of California
OSTI Identifier:
35063
Patent Number(s):
5,405,747
Application Number:
PAN: 8-208,506
Assignee:
Univ. of California Office of Technology Transfer, Alameda, CA (United States)
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 11 Apr 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; RNA; MOLECULAR STRUCTURE; DNA; NUCLEOTIDES

Citation Formats

Jett, J H, Keller, R A, Martin, J C, Posner, R G, Marrone, B L, Hammond, M L, and Simpson, D J. Method for rapid base sequencing in DNA and RNA with two base labeling. United States: N. p., 1995. Web.
Jett, J H, Keller, R A, Martin, J C, Posner, R G, Marrone, B L, Hammond, M L, & Simpson, D J. Method for rapid base sequencing in DNA and RNA with two base labeling. United States.
Jett, J H, Keller, R A, Martin, J C, Posner, R G, Marrone, B L, Hammond, M L, and Simpson, D J. Tue . "Method for rapid base sequencing in DNA and RNA with two base labeling". United States.
@article{osti_35063,
title = {Method for rapid base sequencing in DNA and RNA with two base labeling},
author = {Jett, J H and Keller, R A and Martin, J C and Posner, R G and Marrone, B L and Hammond, M L and Simpson, D J},
abstractNote = {A method is described for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand. 4 figures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {4}
}