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Title: Method for rapid base sequencing in DNA and RNA with two base labeling

A method is described for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand. 4 figures.
Inventors:
; ; ; ; ; ;
Issue Date:
OSTI Identifier:
35063
Assignee:
Univ. of California Office of Technology Transfer, Alameda, CA (United States) PTO; SCA: 550200; PA: EDB-95:065965; SN: 95001365515
Patent Number(s):
US 5,405,747/A/
Application Number:
PAN: 8-208,506
Contract Number:
W-7405-ENG-36
Resource Relation:
Other Information: PBD: 11 Apr 1995
Research Org:
University of California
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; RNA; MOLECULAR STRUCTURE; DNA; NUCLEOTIDES

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