Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU
Abstract
A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences. 9 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 335464
- Patent Number(s):
- 5888730
- Application Number:
- PAN: 8-546,452
- Assignee:
- Univ. of California, Oakland, CA (United States)
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 30 Mar 1999
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; Y CHROMOSOME; POLYMERASE CHAIN REACTION; DNA SEQUENCING; GENE AMPLIFICATION; DNA
Citation Formats
Gray, J W, and Weier, H U. Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU. United States: N. p., 1999.
Web.
Gray, J W, & Weier, H U. Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU. United States.
Gray, J W, and Weier, H U. Tue .
"Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU". United States.
@article{osti_335464,
title = {Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU},
author = {Gray, J W and Weier, H U},
abstractNote = {A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences. 9 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {3}
}
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Works referenced in this record:
A human Y-chromosome specific repeated DNA family (DYZ1) consists of a tandem array of pentanucleotides
journal, January 1986
- Nakahori, Yutaka; Mitani, Kounosuke; Yamada, Masao
- Nucleic Acids Research, Vol. 14, Issue 19