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Title: Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU

Abstract

A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences. 9 figs.

Inventors:
;
Issue Date:
Research Org.:
Univ. of California (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
335464
Patent Number(s):
5888730
Application Number:
PAN: 8-546,452
Assignee:
Univ. of California, Oakland, CA (United States)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 30 Mar 1999
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; Y CHROMOSOME; POLYMERASE CHAIN REACTION; DNA SEQUENCING; GENE AMPLIFICATION; DNA

Citation Formats

Gray, J W, and Weier, H U. Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU. United States: N. p., 1999. Web.
Gray, J W, & Weier, H U. Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU. United States.
Gray, J W, and Weier, H U. Tue . "Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU". United States.
@article{osti_335464,
title = {Y chromosome specific nucleic acid probe and method for identifying the Y chromosome in SITU},
author = {Gray, J W and Weier, H U},
abstractNote = {A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences. 9 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {3}
}

Works referenced in this record:

A human Y-chromosome specific repeated DNA family (DYZ1) consists of a tandem array of pentanucleotides
journal, January 1986