Rapid method to detect duplex formation in sequencing by hybridization methods
Abstract
A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided. A plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex. Each duplex facilitates intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface and exposing the light-sensitive fluid to a light pattern. This causes the fluid exposed to the light to coalesce into discrete units and adhere to the surface. This places each of the units in contact with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units. 13 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Chicago, IL (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 321270
- Patent Number(s):
- 5861247
- Application Number:
- PAN: 8-592,120
- Assignee:
- Univ. of Chicago, IL (United States)
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 19 Jan 1999
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; OLIGONUCLEOTIDES; DNA HYBRIDIZATION; FLUORESCENCE; MOLECULES
Citation Formats
Mirzabekov, A D, Timofeev, E N, Florentiev, V L, and Kirillov, E V. Rapid method to detect duplex formation in sequencing by hybridization methods. United States: N. p., 1999.
Web.
Mirzabekov, A D, Timofeev, E N, Florentiev, V L, & Kirillov, E V. Rapid method to detect duplex formation in sequencing by hybridization methods. United States.
Mirzabekov, A D, Timofeev, E N, Florentiev, V L, and Kirillov, E V. Tue .
"Rapid method to detect duplex formation in sequencing by hybridization methods". United States.
@article{osti_321270,
title = {Rapid method to detect duplex formation in sequencing by hybridization methods},
author = {Mirzabekov, A D and Timofeev, E N and Florentiev, V L and Kirillov, E V},
abstractNote = {A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided. A plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex. Each duplex facilitates intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface and exposing the light-sensitive fluid to a light pattern. This causes the fluid exposed to the light to coalesce into discrete units and adhere to the surface. This places each of the units in contact with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units. 13 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}