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Title: Rapid method to detect duplex formation in sequencing by hybridization methods

Abstract

A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided. A plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex. Each duplex facilitates intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface and exposing the light-sensitive fluid to a light pattern. This causes the fluid exposed to the light to coalesce into discrete units and adhere to the surface. This places each of the units in contact with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units. 13 figs.

Inventors:
; ; ;
Issue Date:
Research Org.:
Univ. of Chicago, IL (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
321270
Patent Number(s):
5861247
Application Number:
PAN: 8-592,120
Assignee:
Univ. of Chicago, IL (United States)
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 19 Jan 1999
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; OLIGONUCLEOTIDES; DNA HYBRIDIZATION; FLUORESCENCE; MOLECULES

Citation Formats

Mirzabekov, A D, Timofeev, E N, Florentiev, V L, and Kirillov, E V. Rapid method to detect duplex formation in sequencing by hybridization methods. United States: N. p., 1999. Web.
Mirzabekov, A D, Timofeev, E N, Florentiev, V L, & Kirillov, E V. Rapid method to detect duplex formation in sequencing by hybridization methods. United States.
Mirzabekov, A D, Timofeev, E N, Florentiev, V L, and Kirillov, E V. Tue . "Rapid method to detect duplex formation in sequencing by hybridization methods". United States.
@article{osti_321270,
title = {Rapid method to detect duplex formation in sequencing by hybridization methods},
author = {Mirzabekov, A D and Timofeev, E N and Florentiev, V L and Kirillov, E V},
abstractNote = {A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided. A plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex. Each duplex facilitates intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface and exposing the light-sensitive fluid to a light pattern. This causes the fluid exposed to the light to coalesce into discrete units and adhere to the surface. This places each of the units in contact with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units. 13 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}