DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase

Abstract

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS){sup {epsilon}}N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the {epsilon}-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed. 8 figs.

Inventors:
Issue Date:
Research Org.:
Univ. of Kentucky, Lexington, KY (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
321268
Patent Number(s):
5866394
Application Number:
PAN: 8-741,931
Assignee:
Univ. of Kentucky Research Foundation, Lexington, KY (United States)
DOE Contract Number:  
FG05-92ER20075
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 2 Feb 1999
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; TRANSFERASES; GENE REGULATION; PLANTS; GENETIC ENGINEERING; GENES; DNA SEQUENCING

Citation Formats

Houtz, R L. Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase. United States: N. p., 1999. Web.
Houtz, R L. Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase. United States.
Houtz, R L. Tue . "Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase". United States.
@article{osti_321268,
title = {Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase},
author = {Houtz, R L},
abstractNote = {The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS){sup {epsilon}}N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the {epsilon}-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed. 8 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 02 00:00:00 EST 1999},
month = {Tue Feb 02 00:00:00 EST 1999}
}

Patent:
Search for the full text at the U.S. Patent and Trademark Office Note: You will be redirected to the USPTO site, which may require a pop-up blocker to be deactivated to view the patent. If so, you will need to manually turn off your browser's pop-up blocker, typically found within the browser settings. (See DOE Patents FAQs for more information.)

Save / Share:

Works referenced in this record:

Affinity Purification of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit ϵN-Methyltransferase
journal, August 1995


Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes
journal, August 1988


Post-translational modifications in the large subunit of ribulose bisphosphate carboxylase/oxygenase.
journal, March 1989


Light-regulated methylation of chloroplast proteins.
journal, July 1987


Cloning and developmental expression of the sucrose-phosphate-synthase gene from spinach
journal, June 1993


Partial Purification and Characterization of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit ε N -Methyltransferase
journal, November 1991


Protein Methylation in Pea Chloroplasts
journal, July 1990