Method for the production of dicarboxylic acids
Abstract
The present invention is an economical fermentation method for the production of carboxylic acids comprising the steps of (a) inoculating a medium having a carbon source with a carboxylic acid-producing organism; (b) incubating the carboxylic acid-producing organism in an aerobic atmosphere to promote rapid growth of the organism thereby increasing the biomass of the organism; (c) controllably releasing oxygen to maintain the aerobic atmosphere; (d) controllably feeding the organism having increased biomass with a solution containing the carbon source to maintain the concentration of the carbon source within the medium of about 0.5 g/l up to about 1 g/l; (e) depriving the aerobic atmosphere of oxygen to produce an anaerobic atmosphere to cause the organism to undergo anaerobic metabolism; (f) controllably feeding the organism having increased biomass a solution containing the carbon source to maintain the concentration of the carbon source within the medium of {>=}1 g/l; and (g) converting the carbon source to carboxylic acids using the anaerobic metabolism of the organism. 7 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 321241
- Patent Number(s):
- 5869301
- Application Number:
- PAN: 8-792,655
- Assignee:
- Lockhead Martin Energy Research Corp., Oak Ridge, TN (United States)
- DOE Contract Number:
- W-31109-ENG-38; AC05-96OR22464
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 9 Feb 1999
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; DICARBOXYLIC ACIDS; BIOSYNTHESIS; MICROORGANISMS; AEROBIC CONDITIONS; ANAEROBIC DIGESTION
Citation Formats
Nghiem, N P, Donnelly, M, Millard, C S, and Stols, L. Method for the production of dicarboxylic acids. United States: N. p., 1999.
Web.
Nghiem, N P, Donnelly, M, Millard, C S, & Stols, L. Method for the production of dicarboxylic acids. United States.
Nghiem, N P, Donnelly, M, Millard, C S, and Stols, L. Tue .
"Method for the production of dicarboxylic acids". United States.
@article{osti_321241,
title = {Method for the production of dicarboxylic acids},
author = {Nghiem, N P and Donnelly, M and Millard, C S and Stols, L},
abstractNote = {The present invention is an economical fermentation method for the production of carboxylic acids comprising the steps of (a) inoculating a medium having a carbon source with a carboxylic acid-producing organism; (b) incubating the carboxylic acid-producing organism in an aerobic atmosphere to promote rapid growth of the organism thereby increasing the biomass of the organism; (c) controllably releasing oxygen to maintain the aerobic atmosphere; (d) controllably feeding the organism having increased biomass with a solution containing the carbon source to maintain the concentration of the carbon source within the medium of about 0.5 g/l up to about 1 g/l; (e) depriving the aerobic atmosphere of oxygen to produce an anaerobic atmosphere to cause the organism to undergo anaerobic metabolism; (f) controllably feeding the organism having increased biomass a solution containing the carbon source to maintain the concentration of the carbon source within the medium of {>=}1 g/l; and (g) converting the carbon source to carboxylic acids using the anaerobic metabolism of the organism. 7 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {2}
}
Works referenced in this record:
Carbon metabolism regulates expression of the pfl (pyruvate formate-lyase) gene in Escherichia coli.
journal, January 1991
- Rasmussen, L. J.; Møller, P. L.; Atlung, T.
- Journal of Bacteriology, Vol. 173, Issue 20