CRISPR/Cas activity assays and compositions thereof
Abstract
The present invention relates, in part, to methods for detecting nuclease activity, such as the activity of Cas nucleases. Also described herein are compositions for conducting assays, as well as methods for conducting assays in the presence of test compounds.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 2293823
- Patent Number(s):
- 11807877
- Application Number:
- 16/360,946
- Assignee:
- National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- NA0003525
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 03/21/2019
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Seamon, Kyle Jeffrey, Harmon, Brooke Nicole, Schoeniger, Joseph S., and Light, Yooli Kim. CRISPR/Cas activity assays and compositions thereof. United States: N. p., 2023.
Web.
Seamon, Kyle Jeffrey, Harmon, Brooke Nicole, Schoeniger, Joseph S., & Light, Yooli Kim. CRISPR/Cas activity assays and compositions thereof. United States.
Seamon, Kyle Jeffrey, Harmon, Brooke Nicole, Schoeniger, Joseph S., and Light, Yooli Kim. Tue .
"CRISPR/Cas activity assays and compositions thereof". United States. https://www.osti.gov/servlets/purl/2293823.
@article{osti_2293823,
title = {CRISPR/Cas activity assays and compositions thereof},
author = {Seamon, Kyle Jeffrey and Harmon, Brooke Nicole and Schoeniger, Joseph S. and Light, Yooli Kim},
abstractNote = {The present invention relates, in part, to methods for detecting nuclease activity, such as the activity of Cas nucleases. Also described herein are compositions for conducting assays, as well as methods for conducting assays in the presence of test compounds.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2023},
month = {11}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Engineering of CRISPR-Cas12b for human genome editing
journal, January 2019
- Strecker, Jonathan; Jones, Sara; Koopal, Balwina
- Nature Communications, Vol. 10, Issue 1
Conductance valve and pressure-to-conductance transducer method and apparatus
patent, January 2005
- Schoeniger, Joseph S.; Cummings, Eric Bryant; Brennan, James S.
- US Patent Document 6,843,272
Therapeutic genome engineering via CRISPR ‐Cas systems
journal, February 2017
- Moreno, Ana M.; Mali, Prashant
- WIREs Systems Biology and Medicine, Vol. 9, Issue 4
Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
journal, September 2017
- Chen, Janice S.; Dagdas, Yavuz S.; Kleinstiver, Benjamin P.
- Nature, Vol. 550, Issue 7676
Method and apparatus for enhanced evanescent fluorescence and color filtering using a high refractive index thin film coating
patent, February 2001
- Kao, Hung Pin; Schoeniger, Joseph S.; Yang, Nancy Y. C.
- US Patent Document 6,188,812
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
journal, January 2014
- Sternberg, Samuel H.; Redding, Sy; Jinek, Martin
- Nature, Vol. 507, Issue 7490
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
journal, October 2017
- Kulcsár, Péter István; Tálas, András; Huszár, Krisztina
- Genome Biology, Vol. 18, Issue 1
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
journal, January 2014
- Fu, Yanfang; Sander, Jeffry D.; Reyon, Deepak
- Nature Biotechnology, Vol. 32, Issue 3
Electrokinetically pumped high pressure sprays
patent, December 2002
- Schoeniger, Joseph S.; Paul, Phillip H.; Schoeniger, Luke
- US Patent Document 6,495,015
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
journal, January 2016
- Kleinstiver, Benjamin P.; Pattanayak, Vikram; Prew, Michelle S.
- Nature, Vol. 529, Issue 7587
DNA targeting specificity of RNA-guided Cas9 nucleases
journal, July 2013
- Hsu, Patrick D.; Scott, David A.; Weinstein, Joshua A.
- Nature Biotechnology, Vol. 31, Issue 9, p. 827-832
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
journal, February 2018
- Hu, Johnny H.; Miller, Shannon M.; Geurts, Maarten H.
- Nature, Vol. 556, Issue 7699
Potential pitfalls of CRISPR/Cas9-mediated genome editing
journal, November 2015
- Peng, Rongxue; Lin, Guigao; Li, Jinming
- The FEBS Journal, Vol. 283, Issue 7
CasX enzymes comprise a distinct family of RNA-guided genome editors
journal, February 2019
- Liu, Jun-Jie; Orlova, Natalia; Oakes, Benjamin L.
- Nature, Vol. 566, Issue 7743
Development of single-tube nested real-time PCR assays with long internally quenched probes for detection of norovirus genogroup II
journal, January 2016
- Xia, Hongyan; Gravelsina, Sabine; Öhrmalm, Christina
- BioTechniques, Vol. 60, Issue 1
Single particle electrochemical sensors and methods of utilization
patent, April 2006
- Schoeniger, Joseph S.; Flounders, Albert W.; Hughes, Robert C.
- US Patent Document 7,022,287
CRISPR-based RNA editing: diagnostic applications and therapeutic options
journal, January 2019
- Gulei, Diana; Raduly, Lajos; Berindan-Neagoe, Ioana
- Expert Review of Molecular Diagnostics, Vol. 19, Issue 2
Linear peptides that mimic structural epitopes for antibody assays
patent, April 2019
- Schoeniger, Joseph S.; Anderson, Peter
- US Patent Document 10,267,788
Diversity, classification and evolution of CRISPR-Cas systems
journal, June 2017
- Koonin, Eugene V.; Makarova, Kira S.; Zhang, Feng
- Current Opinion in Microbiology, Vol. 37
In vivo genome editing using Staphylococcus aureus Cas9
journal, April 2015
- Ran, F. Ann; Cong, Le; Yan, Winston X.
- Nature, Vol. 520, Issue 7546
Conformational control of DNA target cleavage by CRISPR–Cas9
journal, October 2015
- Sternberg, Samuel H.; LaFrance, Benjamin; Kaplan, Matias
- Nature, Vol. 527, Issue 7576
CRISPR/Cas9-mediated genome editing via postnatal administration of AAV vector cures haemophilia B mice
journal, June 2017
- Ohmori, Tsukasa; Nagao, Yasumitsu; Mizukami, Hiroaki
- Scientific Reports, Vol. 7, Issue 1
Potent Inhibition of Human Apurinic/Apyrimidinic Endonuclease 1 by Arylstibonic Acids
journal, November 2007
- Seiple, Lauren A.; Cardellina, John H.; Akee, Rhone
- Molecular Pharmacology, Vol. 73, Issue 3
Effect of Genome Size on AAV Vector Packaging
journal, January 2010
- Wu, Zhijian; Yang, Hongyan; Colosi, Peter
- Molecular Therapy, Vol. 18, Issue 1
Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
journal, September 2016
- Singh, Digvijay; Sternberg, Samuel H.; Fei, Jingyi
- Nature Communications, Vol. 7, Issue 1
RNA-Guided Human Genome Engineering via Cas9
journal, January 2013
- Mali, P.; Yang, L.; Esvelt, K. M.
- Science, Vol. 339, Issue 6121, p. 823-826
Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA
journal, March 2016
- Mekler, Vladimir; Minakhin, Leonid; Semenova, Ekaterina
- Nucleic Acids Research, Vol. 44, Issue 6
Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA
journal, August 2014
- Long, C.; McAnally, J. R.; Shelton, J. M.
- Science, Vol. 345, Issue 6201
Mechanism of duplex DNA destabilization by RNA-guided Cas9 nuclease during target interrogation
journal, May 2017
- Mekler, Vladimir; Minakhin, Leonid; Severinov, Konstantin
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 21
CRISPR RNA-Dependent Binding and Cleavage of Endogenous RNAs by the Campylobacter jejuni Cas9
journal, March 2018
- Dugar, Gaurav; Leenay, Ryan T.; Eisenbart, Sara K.
- Molecular Cell, Vol. 69, Issue 5
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering
journal, May 2013
- Wang, Haoyi; Yang, Hui; Shivalila, Chikdu S.
- Cell, Vol. 153, Issue 4, p. 910-918
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
journal, October 2015
- Zetsche, Bernd; Gootenberg, Jonathan S.; Abudayyeh, Omar O.
- Cell, Vol. 163, Issue 3, p. 759-771
Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing
journal, November 2018
- Tang, Yuyi; Fu, Yan
- Cell & Bioscience, Vol. 8, Issue 1
Programmed DNA destruction by miniature CRISPR-Cas14 enzymes
journal, October 2018
- Harrington, Lucas B.; Burstein, David; Chen, Janice S.
- Science, Vol. 362, Issue 6416
A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
journal, June 2012
- Jinek, M.; Chylinski, K.; Fonfara, I.
- Science, Vol. 337, Issue 6096, p. 816-821
CRISPR Effector System Based Diagnostics
patent-application, October 2018
- Abudayyeh, Omar; Collins, James Joseph; Gootenberg, Jonathan
- US Patent Application 15/922576; 20180298445
Methods and materials for deconstruction of biomass for biofuels production
patent, May 2015
- Schoeniger, Joseph S.; Hadi, Masood Zia
- US Patent Document 9,024,111
In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration
journal, November 2016
- Suzuki, Keiichiro; Tsunekawa, Yuji; Hernandez-Benitez, Reyna
- Nature, Vol. 540, Issue 7631
Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
journal, September 2012
- Gasiunas, G.; Barrangou, R.; Horvath, P.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 39, p. E2579-E2586
Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics
journal, July 2017
- Rose, John C.; Stephany, Jason J.; Valente, William J.
- Nature Methods, Vol. 14, Issue 9
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
journal, December 2014
- Tsai, Shengdar Q.; Zheng, Zongli; Nguyen, Nhu T.
- Nature Biotechnology, Vol. 33, Issue 2
In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy
journal, December 2015
- Nelson, C. E.; Hakim, C. H.; Ousterout, D. G.
- Science, Vol. 351, Issue 6271
Inhibition Mechanism of an Anti-CRISPR Suppressor AcrIIA4 Targeting SpyCas9
journal, July 2017
- Yang, Hui; Patel, Dinshaw J.
- Molecular Cell, Vol. 67, Issue 1
The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit
journal, October 2017
- Murugan, Karthik; Babu, Kesavan; Sundaresan, Ramya
- Molecular Cell, Vol. 68, Issue 1
Versatile High-Throughput Fluorescence Assay for Monitoring Cas9 Activity
journal, April 2018
- Seamon, Kyle J.; Light, Yooli K.; Saada, Edwin A.
- Analytical Chemistry, Vol. 90, Issue 11
Evolution and classification of the CRISPR–Cas systems
journal, May 2011
- Makarova, Kira S.; Haft, Daniel H.; Barrangou, Rodolphe
- Nature Reviews Microbiology, Vol. 9, Issue 6, p. 467-477
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis
journal, August 2013
- Hou, Z.; Zhang, Y.; Propson, N. E.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 39
Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9
journal, December 2013
- Wu, Yuxuan; Liang, Dan; Wang, Yinghua
- Cell Stem Cell, Vol. 13, Issue 6
Selective disruption of an oncogenic mutant allele by CRISPR/Cas9 induces efficient tumor regression
journal, May 2017
- Koo, Taeyoung; Yoon, A-Rum; Cho, Hee-Yeon
- Nucleic Acids Research, Vol. 45, Issue 13
Electrokinetically pumped high pressure sprays
patent, November 2005
- Schoeniger, Joseph S.; Paul, Phillip H.; Schoeniger, Luke
- US Patent Document 6,960,285
Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis: CRISPR-Cas Genome Editing in Xenopus
journal, November 2013
- Nakayama, Takuya; Fish, Margaret B.; Fisher, Marilyn
- genesis, Vol. 51, Issue 12
Electrokinetic concentration of charged molecules
patent, August 2002
- Singh, Anup K.; Neyer, David W.; Schoeniger, Joseph S.
- US Patent Document 6,428,666
Carriers for Plasmid and RNP Delivery in the Treatment of Cancer and Other Disease States
patent-application, February 2018
- Brinker, Charles Jeffrey; Carnes, Eric C.; Ashley, Carlee Erin
- US Patent Application 15/474810; 20180049984
A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice
journal, February 2016
- Yang, Yang; Wang, Lili; Bell, Peter
- Nature Biotechnology, Vol. 34, Issue 3
Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells
journal, June 2016
- Kleinstiver, Benjamin P.; Tsai, Shengdar Q.; Prew, Michelle S.
- Nature Biotechnology, Vol. 34, Issue 8
Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28
journal, February 2017
- Smargon, Aaron A.; Cox, David B. T.; Pyzocha, Neena K.
- Molecular Cell, Vol. 65, Issue 4
RNA-dependent RNA targeting by CRISPR-Cas9
journal, January 2018
- Strutt, Steven C.; Torrez, Rachel M.; Kaya, Emine
- eLife, Vol. 7
Inhibition of CRISPR-Cas9 with Bacteriophage Proteins
journal, January 2017
- Rauch, Benjamin J.; Silvis, Melanie R.; Hultquist, Judd F.
- Cell, Vol. 168, Issue 1-2
Promoting Cas9 degradation reduces mosaic mutations in non-human primate embryos
journal, February 2017
- Tu, Zhuchi; Yang, Weili; Yan, Sen
- Scientific Reports, Vol. 7, Issue 1
Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Triggered Isothermal Amplification for Site-Specific Nucleic Acid Detection
journal, January 2018
- Huang, Mengqi; Zhou, Xiaoming; Wang, Huiying
- Analytical Chemistry, Vol. 90, Issue 3
Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization
journal, February 2017
- Senturk, Serif; Shirole, Nitin H.; Nowak, Dawid G.
- Nature Communications, Vol. 8, Issue 1
Delivery Platforms for the Domestication of Algae Plants
patent-application, March 2016
- Carnes, Eric Christopher; Ruffing, Anne; Ashley, Carlee Erin
- US Patent Application 14/871748; 20160090603
A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
journal, November 2018
- Zhou, Wenhua; Hu, Li; Ying, Liming
- Nature Communications, Vol. 9, Issue 1
High‐throughput screens in mammalian cells using the CRISPR‐Cas9 system
journal, March 2015
- Peng, Jingyu; Zhou, Yuexin; Zhu, Shiyou
- The FEBS Journal, Vol. 282, Issue 11
Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification
journal, January 2016
- Zhang, Kaixiang; Deng, Ruijie; Li, Yue
- Chemical Science, Vol. 7, Issue 8
Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
journal, November 2015
- Karvelis, Tautvydas; Gasiunas, Giedrius; Young, Joshua
- Genome Biology, Vol. 16, Issue 1
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
journal, June 2016
- Abudayyeh, Omar O.; Gootenberg, Jonathan S.; Konermann, Silvana
- Science, Vol. 353, Issue 6299
Permanent Alteration of PCSK9 With In Vivo CRISPR-Cas9 Genome Editing
journal, August 2014
- Ding, Qiurong; Strong, Alanna; Patel, Kevin M.
- Circulation Research, Vol. 115, Issue 5
CRISPR Effector System Based Diagnostics
patent-application, September 2018
- Abudayyeh, Omar; Collins, James Joseph; Gootenberg, Jonathan
- US Patent Application 15/922879; 20180274017
RNA-programmed genome editing in human cells
journal, January 2013
- Jinek, Martin; East, Alexandra; Cheng, Aaron
- eLife, Vol. 2, Article No. e00471
NIH Image to ImageJ: 25 years of image analysis
journal, June 2012
- Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W.
- Nature Methods, Vol. 9, Issue 7
Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo
journal, October 2014
- Zuris, John A.; Thompson, David B.; Shu, Yilai
- Nature Biotechnology, Vol. 33, Issue 1
A catalogue of biochemically diverse CRISPR-Cas9 orthologs
journal, November 2020
- Gasiunas, Giedrius; Young, Joshua K.; Karvelis, Tautvydas
- Nature Communications, Vol. 11, Issue 1
Small molecules, big targets: drug discovery faces the protein–protein interaction challenge
journal, April 2016
- Scott, Duncan E.; Bayly, Andrew R.; Abell, Chris
- Nature Reviews Drug Discovery, Vol. 15, Issue 8
A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays
journal, April 1999
- Zhang, J. -H.
- Journal of Biomolecular Screening, Vol. 4, Issue 2
Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase
journal, November 2016
- Cinesi, Cinzia; Aeschbach, Lorène; Yang, Bin
- Nature Communications, Vol. 7, Issue 1
RNA editing with CRISPR-Cas13
journal, October 2017
- Cox, David B. T.; Gootenberg, Jonathan S.; Abudayyeh, Omar O.
- Science, Vol. 358, Issue 6366
A chemical-inducible CRISPR–Cas9 system for rapid control of genome editing
journal, September 2016
- Liu, Kaiwen Ivy; Ramli, Muhammad Nadzim Bin; Woo, Cheok Wei Ariel
- Nature Chemical Biology, Vol. 12, Issue 11
Disabling Cas9 by an anti-CRISPR DNA mimic
journal, July 2017
- Shin, Jiyung; Jiang, Fuguo; Liu, Jun-Jie
- Science Advances, Vol. 3, Issue 7
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
journal, February 2018
- Chen, Janice S.; Ma, Enbo; Harrington, Lucas B.
- Science, Vol. 360, Issue 6387
Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications
journal, November 2017
- Liu, Chang; Zhang, Li; Liu, Hao
- Journal of Controlled Release, Vol. 266
Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition
journal, November 2015
- Kleinstiver, Benjamin P.; Prew, Michelle S.; Tsai, Shengdar Q.
- Nature Biotechnology, Vol. 33, Issue 12
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
journal, August 2013
- Pattanayak, Vikram; Lin, Steven; Guilinger, John P.
- Nature Biotechnology, Vol. 31, Issue 9
Multiplex Genome Engineering Using CRISPR/Cas Systems
journal, January 2013
- Cong, L.; Ran, F. A.; Cox, D.
- Science, Vol. 339, Issue 6121, p. 819-823
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
journal, February 2019
- Kleinstiver, Benjamin P.; Sousa, Alexander A.; Walton, Russell T.
- Nature Biotechnology, Vol. 37, Issue 3
In vivo gene editing in dystrophic mouse muscle and muscle stem cells
journal, December 2015
- Tabebordbar, M.; Zhu, K.; Cheng, J. K. W.
- Science, Vol. 351, Issue 6271
Synthetic Oligonucleotides Inhibit CRISPR-Cpf1-Mediated Genome Editing
journal, December 2018
- Li, Bin; Zeng, Chunxi; Li, Wenqing
- Cell Reports, Vol. 25, Issue 12
Protocells for Plasmid and RNP Delivery in the Treatment of Cancer and Other Disease States
patent-application, February 2018
- Brinker, Charles Jeffrey; Carnes, Eric C.; Ashley, Carlee Erin
- US Patent Application 15/474800; 20180028686
Microfluidic channel fabrication method
patent, April 2001
- Arnold, Don W.; Schoeniger, Joseph S.; Cardinale, Gregory F.
- US Patent Document 6,210,986
Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA
journal, May 2016
- Yamano, Takashi; Nishimasu, Hiroshi; Zetsche, Bernd
- Cell, Vol. 165, Issue 4
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
journal, May 2016
- Oakes, Benjamin L.; Nadler, Dana C.; Flamholz, Avi
- Nature Biotechnology, Vol. 34, Issue 6
Structural determination of intact proteins using mass spectrometry
patent, May 2008
- Kruppa, Gary; Schoeniger, Joseph S.; Young, Malin
- US Patent Document 7,368,290
Ultrasensitive multi-species detection of CRISPR-Cas9 by a portable centrifugal microfluidic platform
journal, January 2019
- Phaneuf, Christopher R.; Seamon, Kyle J.; Eckles, Tyler P.
- Analytical Methods, Vol. 11, Issue 5
RNA targeting with CRISPR–Cas13
journal, October 2017
- Abudayyeh, Omar O.; Gootenberg, Jonathan S.; Essletzbichler, Patrick
- Nature, Vol. 550, Issue 7675
New variants of CRISPR RNA-guided genome editing enzymes
journal, May 2017
- Murovec, Jana; Pirc, Žan; Yang, Bing
- Plant Biotechnology Journal, Vol. 15, Issue 8
Why Hofmeister effects of many salts favor protein folding but not DNA helix formation
journal, April 2010
- Pegram, Laurel M.; Wendorff, Timothy; Erdmann, Robert
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 17
New CRISPR–Cas systems from uncultivated microbes
journal, December 2016
- Burstein, David; Harrington, Lucas B.; Strutt, Steven C.
- Nature, Vol. 542, Issue 7640
Repurposing CRISPR-Cas12b for mammalian genome engineering
journal, November 2018
- Teng, Fei; Cui, Tongtong; Feng, Guihai
- Cell Discovery, Vol. 4, Issue 1
Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency
journal, June 2016
- Zhang, Jian-Ping; Li, Xiao-Lan; Neises, Amanda
- Scientific Reports, Vol. 6, Issue 1
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
journal, January 2016
- Doench, John G.; Fusi, Nicolo; Sullender, Meagan
- Nature Biotechnology, Vol. 34, Issue 2
Efficient genome editing in zebrafish using a CRISPR-Cas system
journal, January 2013
- Hwang, Woong Y.; Fu, Yanfang; Reyon, Deepak
- Nature Biotechnology, Vol. 31, Issue 3, p. 227-229
CRISPR-Cas orthologues and variants: optimizing the repertoire, specificity and delivery of genome engineering tools
journal, June 2017
- Cebrian-Serrano, Alberto; Davies, Benjamin
- Mammalian Genome, Vol. 28, Issue 7-8
Crisper Effector System Based Diagnostic for Malaria Detection
patent-application, October 2018
- Abudayyeh, Omar; Collins, James Joseph; Gootenberg, Jonathan
- US Patent Application 15/952132; 20180305773
Electrokinetic high pressure hydraulic system
patent, June 2003
- Paul, Phillip H.; Rakestraw, David J.; Arnold, Don W.
- US Patent Document 6,572,749
Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid
patent, October 2017
- Koh, Chung-Yan; Light, Yooli Kim; Piccini, Matthew
- US Patent Document 9,803,238
CAS9-based Isothermal Method of Detection of Specific DNA Sequence
patent-application, July 2015
- Carstens, Carsten-Peter
- US Patent Application 14/607781; 20150211058
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
journal, January 2013
- Cho, Seung Woo; Kim, Sojung; Kim, Jong Min
- Nature Biotechnology, Vol. 31, Issue 3
In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
journal, February 2017
- Kim, Eunji; Koo, Taeyoung; Park, Sung Wook
- Nature Communications, Vol. 8, Issue 1
Rationally engineered Cas9 nucleases with improved specificity
journal, December 2015
- Slaymaker, I. M.; Gao, L.; Zetsche, B.
- Science, Vol. 351, Issue 6268
CRISPR Effector System Based Diagnostics
patent-application, November 2018
- Abudayyeh, Omar; Collins, James Joseph; Gootenberg, Jonathan
- US Patent Application 15/917546; 20180340218
Impeding Transcription of Expanded Microsatellite Repeats by Deactivated Cas9
journal, November 2017
- Pinto, Belinda S.; Saxena, Tanvi; Oliveira, Ruan
- Molecular Cell, Vol. 68, Issue 3
CRISPR/Cas9 – Mediated Precise Targeted Integration In Vivo Using a Double Cut Donor with Short Homology Arms
journal, June 2017
- Yao, Xuan; Wang, Xing; Liu, Junlai
- EBioMedicine, Vol. 20
Structure and Engineering of Francisella novicida Cas9
journal, February 2016
- Hirano, Hisato; Gootenberg, Jonathan S.; Horii, Takuro
- Cell, Vol. 164, Issue 5
Electrokinetic high pressure hydraulic system
patent, August 2001
- Paul, Phillip H.; Rakestraw, David J.; Arnold, Don W.
- US Patent Document 6,277,257
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
journal, June 2015
- Kleinstiver, Benjamin P.; Prew, Michelle S.; Tsai, Shengdar Q.
- Nature, Vol. 523, Issue 7561
A Broad-Spectrum Inhibitor of CRISPR-Cas9
journal, September 2017
- Harrington, Lucas B.; Doxzen, Kevin W.; Ma, Enbo
- Cell, Vol. 170, Issue 6
Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection
journal, September 2016
- East-Seletsky, Alexandra; O’Connell, Mitchell R.; Knight, Spencer C.
- Nature, Vol. 538, Issue 7624
Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors
journal, April 2018
- Konermann, Silvana; Lotfy, Peter; Brideau, Nicholas J.
- Cell, Vol. 173, Issue 3
Deciphering, Communicating, and Engineering the CRISPR PAM
journal, January 2017
- Leenay, Ryan T.; Beisel, Chase L.
- Journal of Molecular Biology, Vol. 429, Issue 2
Naturally Occurring Off-Switches for CRISPR-Cas9
journal, December 2016
- Pawluk, April; Amrani, Nadia; Zhang, Yan
- Cell, Vol. 167, Issue 7
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
journal, January 2018
- Marshall, Ryan; Maxwell, Colin S.; Collins, Scott P.
- Molecular Cell, Vol. 69, Issue 1
In Vitro Evaluation of CRISPR/Cas9 Function by an Electrochemiluminescent Assay
journal, August 2016
- Liu, Weipeng; Yu, Huiheng; Zhou, Xiaoming
- Analytical Chemistry, Vol. 88, Issue 17
A thermostable Cas9 with increased lifetime in human plasma
journal, November 2017
- Harrington, Lucas B.; Paez-Espino, David; Staahl, Brett T.
- Nature Communications, Vol. 8, Issue 1
Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
journal, May 2013
- Gratz, Scott J.; Cummings, Alexander M.; Nguyen, Jennifer N.
- Genetics, Vol. 194, Issue 4
Engineered CRISPR-Cas9 nuclease with expanded targeting space
journal, August 2018
- Nishimasu, Hiroshi; Shi, Xi; Ishiguro, Soh
- Science, Vol. 361, Issue 6408
Method for eliminating gas blocking in electrokinetic pumping systems
patent, September 2001
- Arnold, Don W.; Paul, Phillip H.; Schoeniger, Joseph S.
- US Patent Document 6,287,440