Genetically modified bacterial cells and methods useful for producing indigoidine
Abstract
The present invention provides for a genetically modified bacterial host cell capable of producing indigoidine, wherein the host cell comprises a non-ribosomal peptide synthetase (NRPS) that converts glutamine to indigoidine, and the bacterial host cell is reduced in its expression of one or more of the sixteen indicated enzymes.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 2222363
- Patent Number(s):
- 11767521
- Application Number:
- 17/180,633
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 02/19/2021
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Eng, Thomas T., Banerjee, Deepanwita, and Mukhopadhyay, Aindrila. Genetically modified bacterial cells and methods useful for producing indigoidine. United States: N. p., 2023.
Web.
Eng, Thomas T., Banerjee, Deepanwita, & Mukhopadhyay, Aindrila. Genetically modified bacterial cells and methods useful for producing indigoidine. United States.
Eng, Thomas T., Banerjee, Deepanwita, and Mukhopadhyay, Aindrila. Tue .
"Genetically modified bacterial cells and methods useful for producing indigoidine". United States. https://www.osti.gov/servlets/purl/2222363.
@article{osti_2222363,
title = {Genetically modified bacterial cells and methods useful for producing indigoidine},
author = {Eng, Thomas T. and Banerjee, Deepanwita and Mukhopadhyay, Aindrila},
abstractNote = {The present invention provides for a genetically modified bacterial host cell capable of producing indigoidine, wherein the host cell comprises a non-ribosomal peptide synthetase (NRPS) that converts glutamine to indigoidine, and the bacterial host cell is reduced in its expression of one or more of the sixteen indicated enzymes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2023},
month = {9}
}
Works referenced in this record:
The galactose regulon of Escherichia coli
journal, October 1993
- Weickert, Michael J.; Adhya, Sankar
- Molecular Microbiology, Vol. 10, Issue 2
Engineering Robust Production Microbes for Large-Scale Cultivation
journal, June 2019
- Wehrs, Maren; Tanjore, Deepti; Eng, Thomas
- Trends in Microbiology, Vol. 27, Issue 6
Scale-up of industrial microbial processes
journal, June 2018
- Crater, Jason S.; Lievense, Jefferson C.
- FEMS Microbiology Letters, Vol. 365, Issue 13
Mutant phenotypes for thousands of bacterial genes of unknown function
journal, May 2018
- Price, Morgan N.; Wetmore, Kelly M.; Waters, R. Jordan
- Nature, Vol. 557, Issue 7706
Intragenic tandem repeats generate functional variability
journal, August 2005
- Verstrepen, Kevin J.; Jansen, An; Lewitter, Fran
- Nature Genetics, Vol. 37, Issue 9
A Two-Layer Gene Circuit for Decoupling Cell Growth from Metabolite Production
journal, August 2016
- Lo, Tat-Ming; Chng, Si Hui; Teo, Wei Suong
- Cell Systems, Vol. 3, Issue 2
Regulation and function of proline oxidase under nutrient stress
journal, May 2009
- Pandhare, Jui; Donald, Steven P.; Cooper, Sandra K.
- Journal of Cellular Biochemistry, Vol. 107, Issue 4
OECD-FAO Agricultural Outlook 2018-2027
book, January 2018
- , Oecd; Organization, Food and Agriculture
- OECD-FAO Agricultural Outlook
In-silico-driven metabolic engineering of Pseudomonas putida for enhanced production of poly-hydroxyalkanoates
journal, January 2013
- Poblete-Castro, Ignacio; Binger, Danielle; Rodrigues, Andre
- Metabolic Engineering, Vol. 15
Towards lignin consolidated bioprocessing: simultaneous lignin depolymerization and product generation by bacteria
journal, January 2015
- Salvachúa, Davinia; Karp, Eric M.; Nimlos, Claire T.
- Green Chemistry, Vol. 17, Issue 11
Nature’s Palette: The Search for Natural Blue Colorants
journal, July 2014
- Newsome, Andrew G.; Culver, Catherine A.; van Breemen, Richard B.
- Journal of Agricultural and Food Chemistry, Vol. 62, Issue 28
Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol
journal, May 2011
- Yim, Harry; Haselbeck, Robert; Niu, Wei
- Nature Chemical Biology, Vol. 7, Issue 7
Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in Pseudomonas putida Lysine Metabolism
journal, May 2019
- Thompson, Mitchell G.; Blake-Hedges, Jacquelyn M.; Cruz-Morales, Pablo
- mBio, Vol. 10, Issue 3
Enumeration of Smallest Intervention Strategies in Genome-Scale Metabolic Networks
journal, January 2014
- von Kamp, Axel; Klamt, Steffen
- PLoS Computational Biology, Vol. 10, Issue 1
Efficient production of indigoidine in Escherichia coli
journal, June 2015
- Xu, Fuchao; Gage, David; Zhan, Jixun
- Journal of Industrial Microbiology & Biotechnology, Vol. 42, Issue 8
Purification of RNA Using TRIzol (TRI Reagent)
journal, June 2010
- Rio, D. C.; Ares, M.; Hannon, G. J.
- Cold Spring Harbor Protocols, Vol. 2010, Issue 6
Restoration of biofuel production levels and increased tolerance under ionic liquid stress is enabled by a mutation in the essential Escherichia coli gene cydC
journal, October 2018
- Eng, Thomas; Demling, Philipp; Herbert, Robin A.
- Microbial Cell Factories, Vol. 17, Issue 1
GFP tagging of budding yeast chromosomes reveals that protein–protein interactions can mediate sister chromatid cohesion
journal, December 1996
- Straight, Aaron F.; Belmont, Andrew S.; Robinett, Carmen C.
- Current Biology, Vol. 6, Issue 12
High‐quality genome‐scale metabolic modelling of Pseudomonas putida highlights its broad metabolic capabilities
journal, November 2019
- Nogales, Juan; Mueller, Joshua; Gudmundsson, Steinn
- Environmental Microbiology, Vol. 22, Issue 1
Structure and Function of Enzymes of the Leloir Pathway for Galactose Metabolism
journal, August 2003
- Holden, Hazel M.; Rayment, Ivan; Thoden, James B.
- Journal of Biological Chemistry, Vol. 278, Issue 45
Tolerance engineering in bacteria for the production of advanced biofuels and chemicals
journal, August 2015
- Mukhopadhyay, Aindrila
- Trends in Microbiology, Vol. 23, Issue 8
Identification of Isopentenol Biosynthetic Genes from Bacillus subtilis by a Screening Method Based on Isoprenoid Precursor Toxicity
journal, August 2007
- Withers, S. T.; Gottlieb, S. S.; Lieu, B.
- Applied and Environmental Microbiology, Vol. 73, Issue 19, p. 6277-6283
Cloning and Characterization of a Streptomyces Single Module Type Non-ribosomal Peptide Synthetase Catalyzing a Blue Pigment Synthesis
journal, January 2007
- Takahashi, Hitoshi; Kumagai, Takanori; Kitani, Kyoko
- Journal of Biological Chemistry, Vol. 282, Issue 12
Determination of growth-coupling strategies and their underlying principles
journal, August 2019
- Alter, Tobias B.; Ebert, Birgitta E.
- BMC Bioinformatics, Vol. 20, Issue 1
Isoprenoid Drugs, Biofuels, and Chemicals—Artemisinin, Farnesene, and Beyond
book, January 2015
- George, Kevin W.; Alonso-Gutierrez, Jorge; Keasling, Jay D.
- Biotechnology of Isoprenoids
Sustainable bioproduction of the blue pigment indigoidine: Expanding the range of heterologous products in R. toruloides to include non-ribosomal peptides
journal, January 2019
- Wehrs, Maren; Gladden, John M.; Liu, Yuzhong
- Green Chemistry, Vol. 21, Issue 12
Computational metabolic engineering strategies for growth-coupled biofuel production by Synechocystis
journal, December 2016
- Shabestary, Kiyan; Hudson, Elton P.
- Metabolic Engineering Communications, Vol. 3
Instability of repetitive DNA sequences: The role of replication in multiple mechanisms
journal, July 2001
- Bzymek, M.; Lovett, S. T.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 15
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
journal, October 2015
- Zetsche, Bernd; Gootenberg, Jonathan S.; Abudayyeh, Omar O.
- Cell, Vol. 163, Issue 3, p. 759-771
Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production
journal, September 2019
- Wang, Jian; Zhang, Ruihua; Zhang, Yan
- Metabolic Engineering, Vol. 55
Structural and functional analysis of cellular networks with CellNetAnalyzer
journal, January 2007
- Klamt, Steffen; Saez-Rodriguez, Julio; Gilles, Ernst D.
- BMC Systems Biology, Vol. 1, Issue 1
Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans-metabolism
journal, November 2018
- Nikel, Pablo I.; de Lorenzo, Víctor
- Metabolic Engineering, Vol. 50
Engineering microbial biofuel tolerance and export using efflux pumps
journal, January 2011
- Dunlop, Mary J.; Dossani, Zain Y.; Szmidt, Heather L.
- Molecular Systems Biology, Vol. 7, Issue 1
Techno-economic analysis and life-cycle greenhouse gas mitigation cost of five routes to bio-jet fuel blendstocks
journal, January 2019
- Baral, Nawa Raj; Kavvada, Olga; Mendez-Perez, Daniel
- Energy & Environmental Science, Vol. 12, Issue 3
Growth-coupled bioconversion of levulinic acid to butanone
journal, September 2019
- Mehrer, Christopher R.; Rand, Jacqueline M.; Incha, Matthew R.
- Metabolic Engineering, Vol. 55
Growth-coupled overproduction is feasible for almost all metabolites in five major production organisms
journal, June 2017
- von Kamp, Axel; Klamt, Steffen
- Nature Communications, Vol. 8, Issue 1
Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli
journal, September 2010
- Ajikumar, P. K.; Xiao, W.-H.; Tyo, K. E. J.
- Science, Vol. 330, Issue 6000, p. 70-74
Bioprocess scale-up/down as integrative enabling technology: from fluid mechanics to systems biology and beyond
journal, August 2017
- Delvigne, Frank; Takors, Ralf; Mudde, Rob
- Microbial Biotechnology, Vol. 10, Issue 5
Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli
journal, March 2011
- Shen, Claire R.; Lan, Ethan I.; Dekishima, Yasumasa
- Applied and Environmental Microbiology, Vol. 77, Issue 9, p. 2905-2915
Structural and functional aspects of the nonribosomal peptide synthetase condensation domain superfamily: discovery, dissection and diversity
journal, November 2017
- Bloudoff, Kristjan; Schmeing, T. Martin
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1865, Issue 11
Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays
journal, October 2019
- Reis, Alexander C.; Halper, Sean M.; Vezeau, Grace E.
- Nature Biotechnology, Vol. 37, Issue 11
Genome-scale metabolic rewiring improves titers rates and yields of the non-native product indigoidine at scale
journal, October 2020
- Banerjee, Deepanwita; Eng, Thomas; Lau, Andrew K.
- Nature Communications, Vol. 11, Issue 1
Computing complex metabolic intervention strategies using constrained minimal cut sets
journal, March 2011
- Hädicke, Oliver; Klamt, Steffen
- Metabolic Engineering, Vol. 13, Issue 2
Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae
journal, December 2018
- Wehrs, Maren; Prahl, Jan-Philip; Moon, Jadie
- Microbial Cell Factories, Vol. 17, Issue 1
On the feasibility of growth-coupled product synthesis in microbial strains
journal, July 2015
- Klamt, Steffen; Mahadevan, Radhakrishnan
- Metabolic Engineering, Vol. 30
CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
journal, March 2007
- Barrangou, R.; Fremaux, C.; Deveau, H.
- Science, Vol. 315, Issue 5819
Creation and analysis of biochemical constraint-based models using the COBRA Toolbox v.3.0
journal, February 2019
- Heirendt, Laurent; Arreckx, Sylvain; Pfau, Thomas
- Nature Protocols, Vol. 14, Issue 3
ATP drives direct photosynthetic production of 1-butanol in cyanobacteria
journal, April 2012
- Lan, E. I.; Liao, J. C.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 16