Modified biosensors and biocatalysts and methods of use
Abstract
Biosensors including a nucleic acid encoding a PcaU protein, a PobR protein, a CatM protein, a PcaR protein, or a TphR protein are provided. In some examples, the biosensors include a promoter regulated by the sensed molecule operably linked to a reporter gene. The biosensors may be included in a vector or in cells including one or more of the biosensors or vectors. Modified chorismate pyruvate lyase (UbiC) and modified paraoxonase (PON1) proteins including one or more amino acid substitutions are provided. Finally, methods of selecting biocatalysts with increased activity including transforming a library of cells expressing a biosensor with one or more nucleic acids encoding one or more mutations in a gene involved in a biosynthesis pathway, determining activity of the reporter protein; and selecting a cell with increased reporter protein activity as expressing a biocatalyst with increased activity are provided.
- Inventors:
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO)
- OSTI Identifier:
- 2222221
- Patent Number(s):
- 11739318
- Application Number:
- 16/226,474
- Assignee:
- Triad National Security, LLC (Los Alamos, NM)
- DOE Contract Number:
- 89233218CNA000001
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 12/19/2018
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Jha, Ramesh K., Strauss, Charlie E., and Dale, Taraka. Modified biosensors and biocatalysts and methods of use. United States: N. p., 2023.
Web.
Jha, Ramesh K., Strauss, Charlie E., & Dale, Taraka. Modified biosensors and biocatalysts and methods of use. United States.
Jha, Ramesh K., Strauss, Charlie E., and Dale, Taraka. Tue .
"Modified biosensors and biocatalysts and methods of use". United States. https://www.osti.gov/servlets/purl/2222221.
@article{osti_2222221,
title = {Modified biosensors and biocatalysts and methods of use},
author = {Jha, Ramesh K. and Strauss, Charlie E. and Dale, Taraka},
abstractNote = {Biosensors including a nucleic acid encoding a PcaU protein, a PobR protein, a CatM protein, a PcaR protein, or a TphR protein are provided. In some examples, the biosensors include a promoter regulated by the sensed molecule operably linked to a reporter gene. The biosensors may be included in a vector or in cells including one or more of the biosensors or vectors. Modified chorismate pyruvate lyase (UbiC) and modified paraoxonase (PON1) proteins including one or more amino acid substitutions are provided. Finally, methods of selecting biocatalysts with increased activity including transforming a library of cells expressing a biosensor with one or more nucleic acids encoding one or more mutations in a gene involved in a biosynthesis pathway, determining activity of the reporter protein; and selecting a cell with increased reporter protein activity as expressing a biocatalyst with increased activity are provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2023},
month = {8}
}
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Works referenced in this record:
Rosetta comparative modeling for library design: Engineering alternative inducer specificity in a transcription factor: A Comparative Approach for Protein Design
journal, May 2015
- Jha, Ramesh K.; Chakraborti, Subhendu; Kern, Theresa L.
- Proteins: Structure, Function, and Bioinformatics, Vol. 83, Issue 7
Biosensor-based engineering of biosynthetic pathways
journal, December 2016
- Rogers, Jameson K.; Taylor, Noah D.; Church, George M.
- Current Opinion in Biotechnology, Vol. 42
Nucleic acid and amino acid sequences relating to Acinetobacter baumannii for diagnostics and therapeutics
patent, May 2003
- Breton, Gary; Bush, David
- US Patent Document 6,562,958
Engineering an Acinetobacter regulon for biosensing and high-throughput enzyme screening in E. coli via flow cytometry
journal, May 2014
- Jha, Ramesh K.; Kern, Theresa L.; Fox, David T.
- Nucleic Acids Research, Vol. 42, Issue 12
A microbial sensor for organophosphate hydrolysis exploiting an engineered specificity switch in a transcription factor
journal, August 2016
- Jha, Ramesh K.; Kern, Theresa L.; Kim, Youngchang
- Nucleic Acids Research, Vol. 44, Issue 17
Evolution-guided optimization of biosynthetic pathways
journal, December 2014
- Raman, Srivatsan; Rogers, Jameson K.; Taylor, Noah D.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 50, p. 17803-17808
Isolated PON1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage
patent, May 2014
- Tawfik, Dan; Gupta, Rinkoo Devi; Goldsmith, Moshe
- US Patent Document 8,735,124
Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase: Enhanced stereoselective OP hydrolysis by PON1
journal, April 2006
- Amitai, Gabriel; Gaidukov, Leonid; Adani, Rellie
- FEBS Journal, Vol. 273, Issue 9
Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization
journal, December 2003
- Aharoni, A.; Gaidukov, L.; Yagur, S.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 2
Characterization and Modeling of Transcriptional Cross-Regulation in Acinetobacter baylyi ADP1
journal, June 2012
- Zhang, Dayi; Zhao, Yun; He, Yi
- ACS Synthetic Biology, Vol. 1, Issue 7