Polypeptide and yeast cell compositions and methods of using the same
Abstract
Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE; USDA; National Science Foundation (NSF)
- OSTI Identifier:
- 2222170
- Patent Number(s):
- 11725213
- Application Number:
- 17/341,224
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- DOE Contract Number:
- DEB1253634; FC02-07ER64494; l7-CRHF-0-6055
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 06/07/2021
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Hittinger, Chris Todd, and Baker, EmilyClare Patricia. Polypeptide and yeast cell compositions and methods of using the same. United States: N. p., 2023.
Web.
Hittinger, Chris Todd, & Baker, EmilyClare Patricia. Polypeptide and yeast cell compositions and methods of using the same. United States.
Hittinger, Chris Todd, and Baker, EmilyClare Patricia. Tue .
"Polypeptide and yeast cell compositions and methods of using the same". United States. https://www.osti.gov/servlets/purl/2222170.
@article{osti_2222170,
title = {Polypeptide and yeast cell compositions and methods of using the same},
author = {Hittinger, Chris Todd and Baker, EmilyClare Patricia},
abstractNote = {Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2023},
month = {8}
}
Works referenced in this record:
The naturally occurring alleles of MAL1 in Saccharomyces species evolved by various mutagenic processes including chromosomal rearrangement.
journal, September 1988
- Charron, M. J.; Michels, C. A.
- Genetics, Vol. 120, Issue 1
Inheritance of brewing-relevant phenotypes in constructed Saccharomyces cerevisiae × Saccharomyces eubayanus hybrids
journal, April 2017
- Krogerus, Kristoffer; Seppänen-Laakso, Tuulikki; Castillo, Sandra
- Microbial Cell Factories, Vol. 16, Issue 1
Characterization of AGT1 encoding a general alpha-glucoside transporter from Saccharomyces
journal, September 1995
- Han, Eun-Kyoung; Cotty, Francis; Sottas, Chantal
- Molecular Microbiology, Vol. 17, Issue 6
Maltotriose Utilization by Industrial Saccharomyces Strains: Characterization of a New Member of the -Glucoside Transporter Family
journal, September 2005
- Salema-Oom, M.; Valadao Pinto, V.; Goncalves, P.
- Applied and Environmental Microbiology, Vol. 71, Issue 9
Characterization of maltotriose transporters from the Saccharomyces eubayanus subgenome of the hybrid Saccharomyces pastorianus lager brewing yeast strain Weihenstephan 34/70
journal, November 2012
- Cousseau, F. E. M.; Alves, S. L.; Trichez, D.
- Letters in Applied Microbiology, Vol. 56, Issue 1
Molecular Analysis of Maltotriose Transport and Utilization by Saccharomycescerevisiae
journal, November 2002
- Day, R. E.; Rogers, P. J.; Dawes, I. W.
- Applied and Environmental Microbiology, Vol. 68, Issue 11
Characterization of the Saccharomyces bayanus-type AGT1 transporter of lager yeast: Saccharomyces bayanus-type AGT1 transporter of lager yeast
journal, July 2012
- Vidgren, Virve; Londesborough, John
- Journal of the Institute of Brewing, Vol. 118, Issue 2
Characterization and Functional Analysis of the MAL and MPH Loci for Maltose Utilization in Some Ale and Lager Yeast Strains
journal, December 2005
- Vidgren, V.; Ruohonen, L.; Londesborough, J.
- Applied and Environmental Microbiology, Vol. 71, Issue 12
The maltose permease encoded by the MAL61 gene of Saccharomyces cerevisiae exhibits both sequence and structural homology to other sugar transporters.
journal, November 1989
- Cheng, Q.; Michels, C. A.
- Genetics, Vol. 123, Issue 3
The Thr 505 and Ser 557 residues of the AGT1-encoded α-glucoside transporter are critical for maltotriose transport in Saccharomyces cerevisiae
journal, April 2008
- Smit, A.; Moses, S. G.; Pretorius, I. S.
- Journal of Applied Microbiology, Vol. 104, Issue 4
Alternative Saccharomyces interspecies hybrid combinations and their potential for low-temperature wort fermentation : Alternative Saccharomyces interspecies hybrids for brewing
journal, August 2017
- Nikulin, Jarkko; Krogerus, Kristoffer; Gibson, Brian
- Yeast, Vol. 35, Issue 1
Regulations of sugar transporters: insights from yeast
journal, March 2013
- Horák, J.
- Current Genetics, Vol. 59, Issue 1-2
Structure and function of facultative sugar transporters
journal, August 1999
- Barrett, Michael P.; Walmsleyt, Adrian R.; Gould, Gwyn W.
- Current Opinion in Cell Biology, Vol. 11, Issue 4
Genomic analysis of a key innovation in an experimental Escherichia coli population
journal, September 2012
- Blount, Zachary D.; Barrick, Jeffrey E.; Davidson, Carla J.
- Nature, Vol. 489, Issue 7417
Structural, Physiological and Regulatory Analysis of Maltose Transporter Genes in Saccharomyces eubayanus CBS 12357T
journal, August 2018
- Brickwedde, Anja; Brouwers, Nick; van den Broek, Marcel
- Frontiers in Microbiology, Vol. 9
Genetically Engineered Yeast Cell Having Increased Nadph Production, Method of Increasing Nadph Level in Yeast Cell, Method of Preparing Yeast Cell, and Method of Producing Lactate Using Yeast Cell
patent-application, November 2016
- Jung, Yukyung; Cho, Kwangmyung; Kim, Jinha
- US Patent Application 15/066775; 20160340698
Some controls on oligosaccharide utilization by yeasts: The physiological basis of the Kluyver effect
journal, December 1992
- Barnett, J.
- FEMS Microbiology Letters, Vol. 100, Issue 1-3
Characterization of the putative maltose transporters encoded by YDL247w and YJR160c
journal, January 2002
- Day, Rachel E.; Higgins, Vincent J.; Rogers, Peter J.
- Yeast, Vol. 19, Issue 12
Intracellular Maltose Is Sufficient To Induce
MAL
Gene Expression in
Saccharomyces cerevisiae
journal, October 2002
- Wang, Xin; Bali, Mehtap; Medintz, Igor
- Eukaryotic Cell, Vol. 1, Issue 5
Evolutionary Engineering in Chemostat Cultures for Improved Maltotriose Fermentation Kinetics in Saccharomyces pastorianus Lager Brewing Yeast
journal, September 2017
- Brickwedde, Anja; van den Broek, Marcel; Geertman, Jan-Maarten A.
- Frontiers in Microbiology, Vol. 8
Prolonged Maltose-Limited Cultivation of Saccharomyces cerevisiae Selects for Cells with Improved Maltose Affinity and Hypersensitivity
journal, April 2004
- Jansen, M. L. A.; Daran-Lapujade, P.; de Winde, J. H.
- Applied and Environmental Microbiology, Vol. 70, Issue 4
Maltose Transport by Brewer's Yeasts in Brewer's Wort
journal, January 2003
- Rautio, Jari; Londesborough, John
- Journal of the Institute of Brewing, Vol. 109, Issue 3
Improvement of maltotriose fermentation by Saccharomyces cerevisiae
journal, October 2006
- Stambuk, B. U.; Alves, S. L.; Hollatz, C.
- Letters in Applied Microbiology, Vol. 43, Issue 4
Residue mutations and their impact on protein structure and function: detecting beneficial and pathogenic changes
journal, January 2013
- Studer, Romain A.; Dessailly, Benoit H.; Orengo, Christine A.
- Biochemical Journal, Vol. 449, Issue 3
Repeated batch fermentation from raw starch using a maltose transporter and amylase expressing diploid yeast strain
journal, February 2010
- Yamakawa, Syun-ichi; Yamada, Ryosuke; Tanaka, Tsutomu
- Applied Microbiology and Biotechnology, Vol. 87, Issue 1
Comparative physiology and fermentation performance of Saaz and Frohberg lager yeast strains and the parental species Saccharomyces eubayanus : Fermentaton performance of Saaz and Frohberg yeast
journal, June 2013
- Gibson, Brian R.; Storgårds, Erna; Krogerus, Kristoffer
- Yeast, Vol. 30, Issue 7
Maltotriose utilization in lager yeast strains:MTT1 encodes a maltotriose transporter
journal, January 2005
- Dietvorst, J.; Londesborough, J.; Steensma, H. Y.
- Yeast, Vol. 22, Issue 10
Improved Fermentation Performance of a Lager Yeast after Repair of Its AGT1 Maltose and Maltotriose Transporter Genes
journal, January 2009
- Vidgren, V.; Huuskonen, A.; Virtanen, H.
- Applied and Environmental Microbiology, Vol. 75, Issue 8
Synthetic Yeast Cells and Methods of Making and Using the Same
patent-application, May 2013
- Alexander, William Gerald; Navarro, David Peris; Hittinger, Christopher Todd
- US Patent Application 15/805950; 20180127784
Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists
patent-application, June 2018
- Hittinger, Christopher Todd; Alexander, William Gerald
- US Patent Application 15/878698; 20180155732
The temperature dependence of maltose transport in ale and lager strains of brewer's yeast: The temperature dependence of maltose transport into yeasts
journal, March 2010
- Vidgren, Virve; Multanen, Jyri-Pekka; Ruohonen, Laura
- FEMS Yeast Research, Vol. 10, Issue 4
Construction of Amylolytic Industrial Brewing Yeast Strain with High Glutathione Content for Manufacturing Beer with Improved Anti-Staling Capability and Flavor
journal, November 2010
- Wang, Jin-Jing
- Journal of Microbiology and Biotechnology, Vol. 20, Issue 11
Constructs and Methods for Genome Editing and Genetic Engineering of Fungi and Protists
patent-application, February 2016
- Hittinger, Christopher Todd; Alexander, William Gerald
- US Patent Application 14/826566; 20160046952
Maltose and maltotriose utilisation by group I strains of the hybrid lager yeast Saccharomyces pastorianus
journal, June 2016
- Magalhães, Frederico; Vidgren, Virve; Ruohonen, Laura
- FEMS Yeast Research, Vol. 16, Issue 5
Glucose-induced inactivation/degradation-resistant transporter gene and use thereof
patent, November 2011
- Hatanaka, Haruyo; Omura, Fumihiko
- US Patent Document 8,053,224
Molecular Analysis of Maltotriose Active Transport and Fermentation by Saccharomyces cerevisiae Reveals a Determinant Role for the AGT1 Permease
journal, January 2008
- Alves, S. L.; Herberts, R. A.; Hollatz, C.
- Applied and Environmental Microbiology, Vol. 74, Issue 5
Developments in sporulation and breeding of brewer's yeast
journal, November 1989
- Bilinski, Carl A.; Casey, Gregory P.
- Yeast, Vol. 5, Issue 6